Bio-Rad MCA1456 CD104抗体,Mouse anti Human CD104 antibody, clone 450-9D-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1456 CD104抗体,Mouse anti Human CD104 antibody, clone 450-9D

2024-10-22

Mouse anti Human CD104 antibody, clone 450-9D

货号：MCA1456,MCA1456T

规格：0.2 mg,25 µg

价格：3315,893

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD104

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：450-9D

荧光染料：Purified

产品介绍
Mouse anti Human CD104 antibody, clone 450-9Drecognizes the human beta4 integrin, also known as CD104. CD104 is a ~205 kDa glycoprotein which associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines.Mouse anti Human CD104 antibody, clone 450-9D recognizes an extracellular epitope on the CD104 molecule.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Mustelid	Expected from Sequence
Ferret	√
Mink	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09%Sodium Azide
Carrier Free	Yes
Immunogen	Purified alpha6 beta4 integrin from A431 cells.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow	√	1/25	1/100
Immunofluorescence	√
Immunohistology - Frozen1	√	1/500	1/1000
Immunohistology - Paraffin	x
Immunoprecipitation	√
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 10⁶cells in 100ul. Histology Positive Control TissueHuman Tonsil
数据
Staining of human tonsil cryostat section with Mouse anti human CD104 antibody, clone 450-9D (MCA1456).
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	Flow	100 Tests
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文献
1. Kennel, S.J.et al.(1990) Second generation monoclonal antibodies to the human integrin alpha 6 beta 4.Hybridoma. 9 (3): 243-55.2. Barbaro, V.et al.(2009) Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes.Mol Vis. 15: 2084-93.3. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.Vet Immunol Immunopathol. 132:109-15.4. Smith, K.et al.(2011) Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action.Immunology. 132: 256-65.5. Alam, H.et al.(2011) Loss of keratins 8 and 18 leads to alterations in α6β4-integrin-mediated signalling and decreased neoplastic progression in an oral-tumour-derived cell line.J Cell Sci. 124: 2096-106.6. Lai-cheong, J.E.et al.(2009) Loss-of-function FERMT1 mutations in kindler syndrome implicate a role for fermitin family homolog-1 in integrin activation.Am J Pathol. 175 (4): 1431-41.7. Groves, R.W.et al.(2010) A homozygous nonsense mutation within the dystonin gene coding for the coiled-coil domain of the epithelial isoform of BPAG1 underlies a new subtype of autosomal recessive epidermolysis bullosa simplex.J Invest Dermatol. 130 (6): 1551-7.8. Aasted, B. & Viuff, B. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.Vet Immunol Immunopathol. 119 (1-2): 27-37.

技术参数

产品应用 IHC;IF;Flow;WB;IP

产品类型 Monoclonal Antibody

推荐用于 IHC;IF;Flow;WB;IP

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Bio-Rad MCA1456 CD104抗体,Mouse anti Human CD104 antibody, clone 450-9D

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