Bio-Rad MCA1455G CD45抗体,Rat anti human CARTILAGE OLIGOMERIC MATRIX protein-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1455G CD45抗体,Rat anti human CARTILAGE OLIGOMERIC MATRIX protein

2024-10-22

Rat anti human CARTILAGE OLIGOMERIC MATRIX protein

货号：MCA1455G

规格：0.2 mg

价格：3120

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：Cartilage Oligomeric Matrix Protein

物种：人

宿主：大鼠

抗体亚型：IgG2a

克隆号：MA37C94 (HC484D1)

荧光染料：Purified

产品介绍
Rat anti Human cartilage oligomeric matrix protein antibody, clone MA37C94recognizes human cartilage oligomeric matrix protein (COMP), otherwise known as thrombospondin-5 (TSP-5). COMP is a 757 amino acid matrix glycoprotein bearing four EGF-like domains, a single TSP C-terminal domain and eight TSP type-3 repeats (UniprotP49747). Defects in the COMP gene can lead to the presence of pseudoachondroplasia or multiple epiphyseal dysplasia (Poseyet al.2014).Rat anti Human cartilage oligomeric matrix protein, clone MA37C94 recognizes an epitope located in the central portion of the molecule and has been described as suitable for use in western blotting (Gagarinaet al.2008) and immunohistochemistry (Grigoriadiset al.2006).
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Immunogen	Human cartilage derived COMP
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
Immunocytochemistry-Paraffin	√
Immunohistology - Frozen	√
Immunoprecipitation	√
Western Blotting	√	1/200	1/2000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
数据
Western blot analysis of recombinant Human cartilage oligomeric matrix protein probed with Rat anti Human Cartilage Oligomeric Matrix Protein (MCA1455G) followed by HRP conjugated Goat anti Rat IgG (STAR72)
文献
1. Kobayashi, M.et al.(2016) Cartilage Oligomeric Matrix Protein Increases in Photodamaged Skin.J Invest Dermatol. 136 (6): 1143-9.2. Milz, S.et al.(2005) An immunohistochemical study of the extracellular matrix of the tarsal plate in the upper eyelid in human beings.J Anat. 206 (1): 37-45.3. Zilkens, C.et al.(2010) Spinning around or stagnation - what do osteoblasts and chondroblasts really like?Eur J Med Res. 15 (1): 35-43.4. Milz, S.et al.(2007) An immunohistochemical study of the triangular fibrocartilage complex of the wrist: regional variations in cartilage phenotype.J Anat. 211 (1): 1-7.5. Inui, S.et al.(2011) Identification and characterization of cartilage oligomeric matrix protein as a novel pathogenic factor in keloids.Am J Pathol. 179 (4): 1951-60.6. Viehöfer, A.F.et al.(2015) The molecular composition of the extracellular matrix of the human iliolumbar ligament.Spine J. 15 (6): 1325-31.7. Nemoto, M.et al.(2013) Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration.J Equine Sci. 24 (2): 17-24.8. Gong, S.D.et al.(2019) Elevated plasma cartilage oligomeric matrix protein (COMP) level are associated with the progression of non-traumatic osteonecrosis of femoral head.Clin Chim Acta. 490: 214-21.

技术参数

产品应用 IHC;ELISA;WB;IP

产品类型 Monoclonal Antibody

推荐用于 IHC;ELISA;WB;IP

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Bio-Rad MCA1455G CD45抗体,Rat anti human CARTILAGE OLIGOMERIC MATRIX protein

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