H3K9me1 Recombinant Rabbit Monoclonal Antibody (9H11L2)

货号：701782

规格：100 µg

价格：4809

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Methylated peptide (Lys9) corresponding to human H

物种：其它

宿主：其它

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.5 mg/mL	用法：	1 µg/mL(ICC);1 µg/mL(IF);1-2 µg/mL(WB)

产品详细信息Since it is highly conserved across species, the antibody may react with many other species.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K9me1 Antibody (701782) in IFImmunofluorescence was performed on fixed and permeabilized SH-SY5Y cells for detection of Histone H3K9me1 using Anti-Histone Histone H3K9me1 Recombinant Rabbit Monoclonal Antibody (Product # 701782, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of Histone H3K9me2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, 1:50). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 594 Phalloidin (Product # A12381, 1:200). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Histone H3K9me2. Panel e) represents control cells with no primary antibody to assess background. H3K9me1 Antibody (701782) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of COLO 205 (Lane 1), COLO 205 nuclear (Lane 2), HCT 116 (Lane 3), HCT 116 nuclear (Lane 4), Jurkat (Lane 5), Jurkat nuclear (Lane 6). The blots were probed with Anti-Histone H3K9me1 Recombinant Rabbit Monoclonal Antibody (Product # 701782, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A clear 17kDa band corresponding to Histone H3K9me1 was observed across cell lines according to the different types of lysates. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5%skimmedmilk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

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