Bio-Rad MCA1366F TCR Gamma/Delta抗体,Hamster anti mouse TCR GAMMA/DELTA:FITC

2024-10-22

Hamster anti mouse TCR GAMMA/DELTA:FITC

货号:MCA1366F

规格:0.1 mg

价格:2576

产品类型:流式抗体

品牌:Bio-Rad

抗原:TCR Gamma/Delta

物种:小鼠

宿主:Armenian hamster仓鼠

抗体亚型:IgG

克隆号:GL3

荧光染料:FITC

产品介绍
Hamster anti Mouse TCR Gamma/Delta antibody, clone GL3reacts with mouse TCR gamma/delta expressing lymphocytes.Hamster anti Mouse TCR Gamma/Delta antibody, clone GL3 has been shown to have depleting activityin vivo(Van der Heydeet al.1995and Skeenet al.1993).
产品详情

Target Species

Mouse

Product FormPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

<0.1% Sodium Azide (NaN3)

0.5% Bovine Serum Albumin

ImmunogenC57BL/6J intra epithelial lymphocytes.
Approx. Protein ConcentrationsIgG concentration 0.1 mg/ml

Fusion Partners

Spleen cells from immunized Armenian hamster were fused with cells of the murine myeloma SP2/0 myeloma cell line.

Max Ex/Em

FluorophoreExcitation Max (nm)Emission Max (nm)

FITC

490525

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x106cells in 100ul.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
同型对照
DescriptionProduct CodeApplicationsPack Size
Hamster (Armenian) IgG Negative Control:FITCMCA2356FFlow0.1mg
相关产品
DescriptionProduct CodeApplicationsPack Size
Mouse Seroblock FcRBUF041AF0.1 mg
Mouse Seroblock FcRBUF041BF0.5 mg
文献
1. Kinoshita, N.et al.(2002) Autocrine IL-15 mediates intestinal epithelial cell death via the activation of neighboring intraepithelial NK cells.J Immunol. 169: 6187-92.2. Skelsey, M.E.et al.(2001) Gamma delta T cells are needed for ocular immune privilege and corneal graft survival.J Immunol. 166: 4327-33.2. Skarstein, K.et al.(1994) Oligoclonality of T cells in salivary glands of autoimmune MRL/lpr mice.Immunology. 81 (4): 497-501.3. van der Heyde, H.C.et al.(1995) Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria.J Immunol. 154 (8): 3985-90.4. Skeen, M.J. & Ziegler, H.K. (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection.J Exp Med. 178 (3): 971-84.5. Karasova, D.et al.(2010) Influence of 5 major Salmonella pathogenicity islands on NK cell depletion in mice infected withSalmonella entericaserovar Enteritidis.BMC Microbiol. 10: 75.6.Laky, K.et al.(2000) Enterocyte expression of interleukin 7 induces development of gammadelta T cells and Peyer's patches.J Exp Med. 191: 1569-80.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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