SOX2 Polyclonal Antibody

货号：48-1400

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.25 mg/mL	用法：	1-3 µg/mL (WB)；1:20 (IHC (P))；2-3 µg/mL (ICC/IF)；5 µg (IP)

靶标信息SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

数据

SOX2 Antibody (48-1400) in ICC/IFImmunofluorescence analysis of SOX2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with Methanol for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOX2 Rabbit Polyclonal Antibody (Product # 48-1400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Cytoskeleton was stained with alpha-Tubulin Monoclonal Antibody (Product # 32-2500, 1 µg/mL) followed by Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A-11032, 1:400). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

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参考文献：

1.Frontiers in oncologyCancer Stem Cell Subpopulations Are Present Within Metastatic Head and Neck Cutaneous Squamous Cell Carcinoma."Published figure using SOX2 polyclonal antibody (Product # 48-1400) in Western Blot"2.PloS oneColon adenocarcinoma-derived cells that express induced-pluripotent stem cell markers possess stem cell function."48-1400 was used in Western Blotting to investigate the expression of a panel of induced pluripotent stem cell (iPSC) markers in primary colon adenocarcinoma (CA)-derived cell lines."3.Molecular systems biologyEndogenous fluctuations of OCT4 and SOX2 bias pluripotent cell fate decisions."Published figure using SOX2 polyclonal antibody (Product # 48-1400) in Western Blot"4.Frontiers in immunologyMaternal Immune Activation Alters Fetal Brain Development and Enhances Proliferation of Neural Precursor Cells in Rats."Published figure using SOX2 polyclonal antibody (Product # 48-1400) in Immunohistochemistry"5.Nature communicationsSustained Wnt/β-catenin signalling causes neuroepithelial aberrations through the accumulation of aPKC at the apical pole."48-1400 was used in immunohistochemistry to report that beta-catenin controls the cell fate and polarity of neuroblasts by modulating the expression and localization of aPKC."

技术参数

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