Bio-Rad MCA1295 IgG2c Heavy Chain抗体,Mouse anti Rat IgG2c Heavy Chain-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1295 IgG2c Heavy Chain抗体,Mouse anti Rat IgG2c Heavy Chain

2024-10-22

Mouse anti Rat IgG2c Heavy Chain

货号：MCA1295

规格：0.5 mg

价格：5712

产品类型：流式抗体

品牌：Bio-Rad

抗原：IgG2c Heavy Chain

物种：大鼠

宿主：小鼠

抗体亚型：IgG2a

克隆号：MARG2c-5

荧光染料：Purified

产品介绍
Mouse anti Rat IgG2c heavy chain antibody, clone MARG2c-5recognizes the heavy chain of rat immunoglobulin gamma 2C as determined by dot blot analysis.
产品详情
Target Species	Rat
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.1% Sodium Azide (NaN3)
Immunogen	IgG2c (Rat strain LOU/c).
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/200	1/1000
ELISA			5ug/ml as coating antibody
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 10⁶cells in 100ulELISAClone MARG2c-5 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with ourHRP-conjugated MARK-1/MARL-15 clone cocktail(MCA1296P) as the detection reagent.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG2a Negative Control	MCA1210	F	100 Tests
文献
1. Manouvriez, P.et al. (1986) Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by ELISA with mouse monoclonal antibodies.Immunobiology 173: 307-8.2. Delriviere, L.D.et al.(1998) Basic anatomical and physiological differences between species should be considered when choosing combinations for use in models of hepatic xenotransplantation: an investigation of the guinea pig-to-rat combination.Transplantation. 66: 112-5.3. Lin, Y.et al.(1999) Accommodated xenografts survive in the presence of anti-donor antibodies and complement that precipitate rejection of naive xenografts.J Immunol. 163: 2850-7.4. Lin, Y.et al.(2000) Long-term survival of hamster hearts in presensitized rats.J Immunol. 164: 4883-92.5. Ueta, H.et al.(2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.Int Immunol. 30 (2): 53-67.Further Reading1. van den Berg, T.K.et al.(2001) Monoclonal antibodies against rat leukocyte surface antigens.Immunol Rev. 184: 109-16.

技术参数

产品应用 E ；F

产品类型单克隆抗体

推荐用于 E ；F

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Bio-Rad MCA1295 IgG2c Heavy Chain抗体,Mouse anti Rat IgG2c Heavy Chain

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