Bio-Rad MCA1335GA CD45抗体,mouse anti PIG SLA CLASS II DQ-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1335GA CD45抗体,mouse anti PIG SLA CLASS II DQ

2024-10-22

mouse anti PIG SLA CLASS II DQ

货号：MCA1335GA

规格：0.1 mg

价格：2185

产品类型：流式抗体

品牌：Bio-Rad

抗原： SLA CLASS II DQ

物种：pig

宿主：小鼠

抗体亚型：IgG1

克隆号：K274.3G8

荧光染料：Purified

产品介绍
Mouse anti Pig SLA Class II DQ antibody, clone K274.3G8recognizes SLA DQ molecules which are expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR.
产品详情
Target Species	Pig
Product Form	Purified IgG - liquid
Species Cross-Reactivity	Target Species	Cross Reactivity
Bovine	√
N.B. Antibody reactivity and working conditions may vary between species.
Preparation	Purified IgG prepared by affinity chromatography on Protein A
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)
Carrier Free	Yes
Immunogen	Porcine peripheral blood lymphocytes
Approx. Protein Concentrations	IgG concentration 1.05 mg/ml
Fusion Partners	Spleen cells from immunized mice were fused with cells of the P3-X63-Ag.653 myeloma cell line
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/10	1/100
Immunohistology - Frozen	√
Immunohistology - Paraffin	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 10⁶cells in 100ul .
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	F	100 Tests/1ml
数据
Staining of porcine peripheral blood lymphocytes with Mouse anti Pig SLA Class II D Q (MCA1335GA) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F).
文献
1. Weesendorp Eet al.(2013) Phenotypic modulation and cytokine profiles of antigen presenting cells by European subtype 1 and 3 porcine reproductive and respiratory syndrome virus strainsin vitroandin vivo.Vet Microbiol. 167 (3-4): 638-50.2. Makala, L.H.et al.(2001) Ontogeny of pig discrete Peyer's patches: expression of surface antigens.J Vet Med Sci. 63 (6): 625-36.3. Facci, M.R.et al.(2010) A comparison between isolated blood dendritic cells and monocyte-derived dendritic cells in pigs.Immunology. 129 (3): 396-405.4. Edamura, K.et al.(2005) Effect of long-term culture on the expression of antigens and adhesion molecule in single porcine pancreatic endocrine cells.Xenotransplantation. 12 (4): 327-32.5. Debeer, S.et al.(2013) Comparative histology and immunohistochemistry of porcine versus human skin.Eur J Dermatol. 23 (4): 456-66.6. Loss, H.et al.(2018) Effects of a pathogenic ETEC strain and a probioticEnterococcus faeciumstrain on the inflammasome response in porcine dendritic cells.Vet Immunol Immunopathol. 203: 78-87.7. Vreman, S.et al.(2018) Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation.Dev Comp Immunol. 84: 361-70.8. LeLuduec, J.B.et al.(2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.Vaccine. 34 (7): 914-22.

技术参数

产品应用 IHC;Flow

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Bio-Rad MCA1335GA CD45抗体,mouse anti PIG SLA CLASS II DQ

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