Bio-Rad MCA1271A647 CD33抗体,mouse anti human CD33:Alexa Fluor® 647-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1271A647 CD33抗体,mouse anti human CD33:Alexa Fluor® 647

2024-10-22

mouse anti human CD33:Alexa Fluor® 647

货号：MCA1271A647,MCA1271A647T

规格：100 Tests/1ml,25 Tests/0.25ml

价格：3247,1165

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD33

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号： WM53

荧光染料：Alexa Fluor 647

产品介绍
Mouse anti Human CD33 antibody, clone WM53recognizes the human CD33 cell surface glycoprotein. This antigen, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin.Mouse anti Human CD33 antibody, clone WM53 immunoprecipitates a protein of ~75 kDa from myeloid cells, a smaller protein of approximately 67 kDa has been observed in immunoprecipitates from lymphoid targets.
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
Immunogen	Human AML cells
Approx. Protein Concentrations	IgG concentration 0.05 mg/ml
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®647	650	665
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
Acknowledgements	The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 10⁶cells or 100ul whole blood.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	F	100 Tests/1ml
相关产品
Description	Product Code	Applications	Pack Size
Human Seroblock	BUF070A	F	50 Test
Human Seroblock	BUF070B	F	200 Test
数据
Figure A. FITC conjugated mouse anti human CD14 (MCA596F) and Alexa647® conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti human CD14 (MCA596F) and Alexa647® conjugated mouse anti human CD33 (MCA1271A647). All experiments performed on red cell lysed Human peripheral blood gated on myeloid cells in the presence of Human SeroBlock (BUF070A).
文献
1. Lajaunias, F.et al.(2005) Constitutive repressor activity of CD33 on human monocytes requires sialic acid recognition and phosphoinositide 3-kinase-mediated intracellular signaling.Eur J Immunol. 35: 243-51.2. Pietschmann, P.et al.(2000) Surface markers and transendothelial migration of dendritic cells from elderly subjects.Exp Gerontol. 35: 213-24.3. Favaloro, E.J.et al. (1993) Differential expression of surface antigens on activated endothelium.Immunol Cell Biol. 71:571-81.4. Yasukawa, T.et al.(2012) Simple detection of surface antigens on living cells by applying distinct cell positioning with negative dielectrophoresis.Anal Chem. 84 (20): 8830-6.5. Hu, Z.et al.(2016) Self-assembled nanoparticles based on folic acid modified carboxymethyl chitosan conjugated with targeting antibodyJ Wuhan Univ of Technol-Mater. Sci. Ed. 31 (2): 446-53.6. Dahl Cet al.(2004) Human mast cells express receptors for IL-3, IL-5 and GM-CSF; a partial map of receptors on human mast cells culturedin vitro.Allergy. 59 (10): 1087-96.7. Vamvakopoulos, J.E. & Green, C. (2003) HMG-CoA reductase inhibition aborts functional differentiation and triggers apoptosis in cultured primary human monocytes: a potential mechanism of statin-mediated vasculoprotection.BMC Cardiovasc Disord. 3: 6.8. Vamvakopoulos, J.et al.(2002) Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.Eur J Immunol. 32 (10): 2988-96.9. Lin, C.W.et al.(2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.Blood. 106 (10): 3567-74.10. McCormack Eet al.(2013) Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.Blood. 121 (7): e34-42.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Bio-Rad MCA1271A647 CD33抗体,mouse anti human CD33:Alexa Fluor® 647

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