Bio-Rad MCA1270A488 CD13抗体,Mouse anti Human CD13:Alexa Fluor 488

2024-10-22

Mouse anti Human CD13:Alexa Fluor 488

货号:MCA1270A488,MCA1270A488T

规格:100 Tests/1ml,25 Tests/0.25ml

价格:3247,1420

产品类型:流式抗体

品牌:Bio-Rad

抗原: CD13

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:WM15

荧光染料:Alexa Fluor 488

产品介绍
Mouse anti Human CD13 antibody, clone WM15recognizes human CD13 also known as aminopeptidase N. CD13 is a single pass type II glycosylated integral membrane protein with a predicted molecular mass of ~110 kDa and an apparent molecular mass of ~150 kDa expressed by granulocytes, monocytes, fibroblasts, endothelial cells and by myeloid leukaemia cells (Bradstocket al.1985). CD13 acts as a major cell surface receptor for group 1 coronoviruses (Breslinet al.2003) which bind to a critical sequence encompassing amino acid residies 288-295 (Kolbet al.1997).CD13 functions as anaminopeptidaseenzyme, a metalloprotease present as both a membrane bound form and also a soluble aminopeptidase N.Mouse anti Human CD13, clone WM15 inhibits infection of cells by human coronavirus (Lachanceet al.1998) but not hepatitis C virus (Koutsoudakiset al.2006) and inhibits aminopeptidase N activity of the CD13 molecule (Asmunet al.1992).
产品详情

Target Species

Human

Species Cross-Reactivity

Target SpeciesCross Reactivity
Rhesus Monkey

N.B. Antibody reactivity and working conditions may vary between species.
Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preparation

  • MCA1270A488: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

  • MCA1270A488T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin

Approx. Protein ConcentrationsIgG concentration 0.05 mg/ml
ImmunogenHuman AML cells.

Fusion Partners

Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line

Max Ex/Em

FluorophoreExcitation Max (nm)Emission Max (nm)

Alexa Fluor®488

495519

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

Neat1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells or 100ul whole blood
同型
DescriptionProduct CodeApplicationsPack Size

Mouse IgG1 Negative Control:Alexa Fluor® 488

MCA928A488

F

100 Tests

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文献
1. Stolzing, A.et al.(2008) Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies.Mech Ageing Dev. 129: 163-73.2. Silk, K.M.et al.(2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.J Biomed Biotechnol. 2012:172420.3. Negussie, A.H.et al.(2010) Synthesis and in vitro evaluation of cyclic NGR peptide targeted thermally sensitive liposome.J Control Release. 143: 265-73.4. Lassnig, C.et al.(2005) Development of a transgenic mouse model susceptible to human coronavirus 229E.Proc Natl Acad Sci U S A. 102 (23): 8275-80.5. Thielitz, A.et al.(2004) Identification of extra- and intracellular alanyl aminopeptidases as new targets to modulate keratinocyte growth and differentiation.Biochem Biophys Res Commun. 321 (4): 795-801.6. McCormack, E.et al.(2013) Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.Blood. 121 (7): e34-42.7. Fiddler, C.A.et al.(2016) The Aminopeptidase CD13 Induces Homotypic Aggregation in Neutrophils and Impairs Collagen Invasion.PLoS One. 11 (7): e0160108.8. Chaturvedi, C.P.et al.(2018) Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.Stem Cells Int. 2018: 6901761.

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