Bio-Rad MCA1269APC CD100,Mouse anti Human CD100:APC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1269APC CD100,Mouse anti Human CD100:APC

2024-10-22

Mouse anti Human CD100:APC

货号：MCA1269APC

规格：100 Tests

价格：4038

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD100

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：A8

荧光染料：APC

产品介绍
Mouse anti Human CD100 antibody, clone A8recognizes human Sepaphorin 4D, also known as CD100, A8, BB18 or GR3. CD100 is a 841 amino acid ~150 kDa homodimeric single pass type 1 transmembrane cell surface glycoprotein acting as a receptor for some members of the plexin family. CD100 is expressed at low levels by peripheral blood T cells, and at higher density on activated T cells and B cells, it is also expresses at high levels in fetal kidney with lower levels in fetal brain, liver and lung (Maestriniet al.1996).
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Cynomolgus monkey	√
Rhesus Monkey	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
Reconstitution	Reconstitute with 1ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin 5%Sucrose
Immunogen	Human PHA activated lymphocytes.
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
APC	650	661
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Prior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat	1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 106cells in 100ul.
数据
Staining of human peripheral blood lymphocytes with Mouse anti Human CD100:APC (MCA1269APC).
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:APC	MCA928APC	F	100 Tests
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文献
1. Aversa, G.G.et al.(1989) Use of monoclonal antibodies to studyin vivoandin vitro-activated lymphocytes.Transplant Proc. 21 (1 Pt 1): 349-50.2. Van Vliet, S. J.et al. (1995) Species cross reactivity (human-monkey-bovine) of the adhesion structure section mAb.Leucocyte Typing V. Oxford University Press p1607-8.3. Mizrahi, S.et al.(2007) CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72.PLoS One. 2 (9): e818.4. Yoshino, N.et al.(2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.Exp Anim. 49 (2): 97-110.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Bio-Rad MCA1269APC CD100,Mouse anti Human CD100:APC

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