Bio-Rad MCA1278 CD45抗体,Rat anti Horse CD2-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1278 CD45抗体,Rat anti Horse CD2

2024-10-22

Rat anti Horse CD2

货号：MCA1278

规格：2 ml

价格：3383

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD2

物种：Horse

宿主：驴

抗体亚型：IgG2a

克隆号：Mac288

荧光染料：Purified

产品介绍
Rat anti Horse CD2 antibody, clone Mac288recognizes equine CD2, a 347 amino acid, single pass type-1 transmembrane glycoprotein containing singleIg-like C2-typeandIg-like V-typedomains.Equine CD2 is expressed by a large proportion of peripheral blood lymphocytes, most T-cells and few B-cells, it is not present on granulocytes. Immunohistochemical staining shows expression in most T cells of lymph node paracortex and occasional staining of lymphocytes in the follicular areas. In the thymus, rat anti horse CD2 antibody, clone Mac288 stains the majority of mature thymocytes in the medulla and at least half of the immature thymocytes in the cortex (Kyddet al.1994). Rat anti Horse CD2 antibody, clone Mac288 recognizes CD2 in Haflinger and Lusitano horse breeds (Ferreira-Diaset al.2005). Rat anti Horse CD2 antibody, clone Mac288 is a reliable pan T-cell marker for analysis of cell mediated immunity in horses infected with equine herpes virus by multi-parameter flow cytometry (Plattet al.2010).
产品详情
Target Species	Horse
Species Cross-Reactivity	Target Species	Cross Reactivity
Pig	X
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Tissue Culture Supernatant - liquid
Preservative Stabilisers	0.1% Sodium Azide
Immunogen	Equine peripheral blood lymphocytes.
Fusion Partners	Spleen cells from immunized LOU Rats were fused with cells of the Y3 Ag1.2.3. myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		1/200
Immunohistology - Frozen	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 50ul of the suggested working dilution to label 7.5 x 10⁵cells in 100ul
数据
Staining of equine peripheral blood lymphocytes with Rat anti Equine CD2 (MCA1278), visualized with Rabbit anti Rat IgG:FITC (STAR17B).
文献
1. Tavernor, A.S.et al.(1994) Expression cloning of an equine T-lymphocyte glycoprotein CD2 cDNA. Structure-based analysis of conserved sequence elements.Eur J Biochem. 219 (3): 969-76.3. Pearson, W.et al.(2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses.Can J Vet Res. 71: 213-7.3. Kydd, J.H. (1989) Maternal immune responses to pregnancy in equids.PhD Thesis (Cantab).4. Platt, R.et al.(2010) Cell-mediated immunity evaluation in foals infected with virulent equine herpesvirus-1 by multi-parameter flow cytometry.Vet Immunol Immunopathol. 135: 275-81.5. Roberto Da Costa, R.P.et al.(2003) Peripheral blood neutrophil function and lymphocyte subpopulations in cycling mares.Reprod Domest Anim. 38: 464-9.6. McCandless, E.E.et al.(2013) Hydrocortisone inhibits IFN-γ production in equine, ovine, and bovine PBMCs.Vet Immunol Immunopathol. 153: 128-33.7. Krakowski, L.et al.(2017) Changes in Blood Lymphocyte Subpopulations and Expression of MHC-II Molecules in Wild Mares Before and After Parturition.J Vet Res. 61 (2): 217-21.8. Siqueira, R.F. and Fernandes, I.R. (2018) Cryopreservation of lymphocytes for immunological studies in horsesPesquisa Veterinária Brasileira. 38 (11): 2019-22.

技术参数

产品应用 Flow;IHC

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Bio-Rad MCA1278 CD45抗体,Rat anti Horse CD2

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