Bio-Rad MCA1212A700,Rat IgG2a Negative Control:Alexa Fluor 700-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1212A700,Rat IgG2a Negative Control:Alexa Fluor 700

2024-10-22

Rat IgG2a Negative Control:Alexa Fluor 700

货号：MCA1212A700

规格：100 Tests/1ml

价格：3120

产品类型：大鼠同型对照

品牌：Bio-Rad

物种：大鼠

宿主：大鼠

抗体亚型：IgG2a

荧光染料：Alexa Fluor 700

产品介绍
at IgG2a Negative Control antibodyis suitable for the assessment of the level of non-specific binding of rat IgG2a monoclonal antibodies to mouse cells.Test results indicate Rat IgG2a Negative Control antibody is also suitable for use as a negative control with canine cells.N.B.This antibody recognizes a human cell surface marker, and therefore is not suitable as a negative control in human cells or cell lines.
产品详情
Target Species	NegativeControl
Product Form	Purified IgG conjugated to Alexa Fluor® 700 - liquid
Approx. Protein Concentrations	IgG concentration 0.05 mg/ml
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1%Bovine Serum Albumin
Immunogen	Human T lymphocytes.
Fusion Partners	Spleen cells from immunized DA rats were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®700	702	723
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		*
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. * It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody. Flow Cytometry Use 10ul of the suggested working dilution to label 106cells in 100ul.
数据
Figure A. A647 conjugated Rat anti Mouse CD45R (MCA1258A647) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. A647 conjugated Rat anti Mouse CD45R (MCA1258A647) and RPE conjugated Rat anti mouse CD40 (MCA1143PE). All experiments performed on mouse splenocytes in the presence of mouse SeroBlock (BUF041A)
文献
1. Sumagin, R.et al.(2008) Leukocyte-endothelial cell interactions are linked to vascular permeability via ICAM-1-mediated signaling.Am J Physiol Heart Circ Physiol. 295: H969-H977.2. Chiu, W.C.et al.(2011) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.J Nutr Biochem. 20: 254-60.3. Guilloteau, L.A.et al.(2003) Nramp1 is not a major determinant in the control ofBrucella melitensisinfection in mice.Infect Immun. 71: 621-8.4. Stapleton, T.W.et al.(2000) Investigation of the regenerative capacity of an acellular porcine medial meniscus for tissue engineering applications.Tissue Eng Part A. 17: 231-42.5. Park, S.W.et al.(2012) A1 adenosine receptor allosteric enhancer PD-81723 protects against renal ischemia-reperfusion injury.Am J Physiol Renal Physiol. 303: F721-32.6. Schmidt, E.P.et al.(2012) The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.Nat Med. 18 (8): 1217-23.7. McConnell, M.J.et al.(2009) H2-K(b) and H2-D(b) regulate cerebellar long-term depression and limit motor learning.Proc Natl Acad Sci U S A. 106: 6784-9.8.Rabadi, M.et al.(2016) Peptidyl arginine deiminase-4-deficient mice are protected against kidney and liver injury after renal ischemia and reperfusion.Am J Physiol Renal Physiol. 311 (2): F437-49.9. Rabadi, M.M.et al.(2019) Peptidyl arginine deiminase-4 exacerbates ischemic AKI by finding NEMO (NFκB Essential Modulator).Am J Physiol Renal Physiol. Apr 03 [Epub ahead of print].10. Han, S.J.et al.(2020) Renal proximal tubular NEMO plays a critical role in ischemic acute kidney injury.JCI Insight. 5 (19)Sep 17 [Epub ahead of print].

技术参数

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Bio-Rad MCA1212A700,Rat IgG2a Negative Control:Alexa Fluor 700

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