IgM Monoclonal Antibody (eB121-15F9), FITC,

货号：11-5890-81,11-5890-82 ,11-5890-85

规格：50 ug,100 ug,500 ug

市场价格：822,1545,1959

产品类型：流式抗体

品牌：eBioscience

抗原：IgM

物种：人

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eB121-15F9

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.Applications Reported: eB121-15F9 has been reported for usein flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs.Applications Tested: The eB121-15F9 antibody has been tested by flow cytometric analysis to detect the surface IgM on mouse B cells and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

数据

IgM Antibody (11-5890-81) in FlowStaining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-42) (left) or 0.25 µg of Anti-Mouse IgM FITC (right). Total viable cells were used for analysis.

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参考文献

1. International immunologyThymic stromal lymphopoietin (TSLP)-induced polyclonal B-cell activation and autoimmunity are mediated by CD4+ T cells and IL-4.2. Science advancesCis-regulatory mechanisms governing stem and progenitor cell transitions.3. Scientific reportsV(D)J recombination process and the Pre-B to immature B-cells transition are altered in Fanca-/-mice.4. Proceedings of the National Academy of Sciences of the United States of AmericaHuman leukemia mutations corrupt but do not abrogate GATA-2 function.5. Nature communicationsZNF506-dependent positive feedback loop regulates H2AX signaling after DNA damage.

技术参数

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