Bio-Rad MCA1212XZ 大鼠IgG2a同型,Rat IgG2a Negative Control:Preservative Free-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1212XZ 大鼠IgG2a同型,Rat IgG2a Negative Control:Preservative Free

2024-10-22

Rat IgG2a Negative Control:Preservative Free

货号：MCA1212XZ

规格：1 mg

价格：8160

产品类型：大鼠同型对照

品牌：Bio-Rad

物种：其它

宿主：大鼠

抗体亚型：IgG2a

荧光染料：Preservative Free

产品介绍
at IgG2a Negative Control antibodyis suitable for the assessment of the level of non-specific binding of rat IgG2a monoclonal antibodies to mouse cells.Test results indicate Rat IgG2a Negative Control antibody is also suitable for use as a negative control with canine cells.N.B.This antibody recognizes a human cell surface marker, and therefore is not suitable as a negative control in human cells or cell lines.
产品详情
Target Species	NegativeControl
Product Form	Purified IgG - liquid
Approx. Protein Concentrations	IgG concentration 1 mg/ml
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	None present
Immunogen	Human T lymphocytes.
Fusion Partners	Spleen cells from immunized DA rats were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		*
ELISA	√
Immunohistology - Frozen	x
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.* It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.
文献
1. Sumagin, R.et al.(2008) Leukocyte-endothelial cell interactions are linked to vascular permeability via ICAM-1-mediated signaling.Am J Physiol Heart Circ Physiol. 295: H969-H977.2. Chiu, W.C.et al.(2011) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.J Nutr Biochem. 20: 254-60.3. Guilloteau, L.A.et al.(2003) Nramp1 is not a major determinant in the control ofBrucella melitensisinfection in mice.Infect Immun. 71: 621-8.4. Stapleton, T.W.et al.(2000) Investigation of the regenerative capacity of an acellular porcine medial meniscus for tissue engineering applications.Tissue Eng Part A. 17: 231-42.5. Park, S.W.et al.(2012) A1 adenosine receptor allosteric enhancer PD-81723 protects against renal ischemia-reperfusion injury.Am J Physiol Renal Physiol. 303: F721-32.6. Schmidt, E.P.et al.(2012) The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis.Nat Med. 18 (8): 1217-23.7. McConnell, M.J.et al.(2009) H2-K(b) and H2-D(b) regulate cerebellar long-term depression and limit motor learning.Proc Natl Acad Sci U S A. 106: 6784-9.8.Rabadi, M.et al.(2016) Peptidyl arginine deiminase-4-deficient mice are protected against kidney and liver injury after renal ischemia and reperfusion.Am J Physiol Renal Physiol. 311 (2): F437-49.9. Rabadi, M.M.et al.(2019) Peptidyl arginine deiminase-4 exacerbates ischemic AKI by finding NEMO (NFκB Essential Modulator).Am J Physiol Renal Physiol. Apr 03 [Epub ahead of print].10. Han, S.J.et al.(2020) Renal proximal tubular NEMO plays a critical role in ischemic acute kidney injury.JCI Insight. 5 (19)Sep 17 [Epub ahead of print].

技术参数

产品应用 ELISA;Flow

产品类型 Negative/Isotype Control

推荐用于 ELISA;Flow

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Bio-Rad MCA1212XZ 大鼠IgG2a同型,Rat IgG2a Negative Control:Preservative Free

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