IFN gamma Monoclonal Antibody (4S.B3), FITC, eBioscience™

货号：11-7319-82,11-7319-81

规格：100 µg,50 µg

价格：2019,1237

产品类型：流式抗体

品牌：Thermo Fisher

抗原：IFN gamma

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号： 4S.B3

荧光染料：FITC

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	1.0µg/test（Flow）

产品详细信息Description: The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.Applications Reported: The 4S.B3 antibody has been reported for use in intracellular staining for flow cytometric analysis.Applications Tested: This 4S.B3 antibody is offered in 2 formats: - µg size: has been tested by intracellular flow cytometric analysis of activated human cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular flow cytometric analysis of activated human cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.仅用于科研。不用于诊断过程。

数据

IFN gamma Antibody (11-7319-82) in FlowIntracellular staining of stimulated normal human peripheral blood cells with Anti-Human CD8a PerCP-eFluor® 710 (Product # 46-0087-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-81) (left) or Anti-Human IFN gamma FITC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. PloS oneSkewed distribution of IL-7 receptor-α-expressing effector memory CD8+ T cells with distinct functional characteristics in oral squamous cell carcinoma."11-7319 was used in Flow cytometry/Cell sorting to demonstrate that IL-7 receptor expression on CD8+ T cells may differentiate the CD45RA+/2 CCR72 effector memory CD8+ T-cell subsets that exhibit distinct functional characteristics and association with tumour progression in OSCC patients."AuthorsLee JJ,Yeh CY,Jung CJ,Chen CW,Du MK,Yu HM,Yang CJ,Lin HY,Sun A,Ko JY,Cheng SJ,Chang YL,Chia JSYear2014SpeciesHuman 2. PloS oneElevated frequencies of circulating Th22 cell in addition to Th17 cell and Th17/Th1 cell in patients with acute coronary syndrome."11-7319 was used in Flow cytometry/Cell sorting to investigate the roles and profiles of T helper cells in the pathogenesis of inflammatory heart diseases."AuthorsZhang L,Wang T,Wang XQ,Du RZ,Zhang KN,Liu XG,Ma DX,Yu S,Su GH,Li ZH,Guan YQ,Du NLYear2014SpeciesHuman 3. PloS oneDysregulation of Th17 cells during the early post-transplant period in patients under calcineurin inhibitor based immunosuppression."11-7319 was used in Flow cytometry/Cell sorting to investigate changes in the immune cell subsets of renal transplant recipients before transplant and during the early posttransplant period."AuthorsChung BH,Kim KW,Kim BM,Piao SG,Lim SW,Choi BS,Park CW,Kim YS,Cho ML,Yang CW4. Journal of immunology (Baltimore, Md. : 1950)Vitamin D receptor gene polymorphisms and HLA DRB1*04 cosegregation in Saudi type 2 diabetes patients."11-7319 was used in Flow cytometry/Cell sorting to show that type 2 diabetes mellitus is likely to be modulated by an HLA/VDR interaction."AuthorsAl-Daghri NM,Al-Attas O,Alokail MS,Alkharfy KM,Draz HM,Agliardi C,Mohammed AK,Guerini FR,Clerici MYear2012SpeciesHuman 5. PLoS pathogensLongevity and composition of cellular immune responses following experimental Plasmodium falciparum malaria infection in humans."11-7319 was used in Flow cytometry/Cell sorting to study cellular immunological responses to sporozoites and asexual blood-stage malaria parasites."AuthorsTeirlinck AC,McCall MB,Roestenberg M,Scholzen A,Woestenenk R,de Mast Q,van der Ven AJ,Hermsen CC,Luty AJ,Sauerwein RW

技术参数

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