CD4 Monoclonal Antibody (YKIX302.9), PE-Cyanine7, eBioscience™

货号：25-5040-41

规格：100 Tests

价格：656

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD4

物种：dog

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号： YKIX302.9

荧光染料：PE-Cy7

产品介绍
Applications	流式细胞分析 (Flow)
Tested Dilution	5 µL (0.06 µg)/test
Publications	View 2 publications
种属反应	Dog
已发表种属	Dog
宿主/亚型	Rat / IgG2a, kappa
推荐的同型对照	Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™
分类	Monoclonal
类型	Antibody
克隆号	YKIX302.9
偶联物	PE-Cyanine7
形式	Liquid
浓度	5 µL/Test
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
内含物	0.09% sodium azide
保存条件	4° C, store in dark, DO NOT FREEZE!

产品详细信息Description: The YKIX302.9 monoclonal antibody reacts with canine CD4, a cell surface receptor expressed on a majority of thymocytes, a subpopulation of mature T cells and dendritic cells. In dogs, CD4 is also highly expressed on neutrophils. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role in T cell development and homeostasis.This YKIX302.9 monoclonal antibody has been reported to block MHC II-restricted T cell responses in vivo and invitro.Applications Reported: This YKIX302.9 antibody has been reported for use in flow cytometric analysis.Applications Tested: This YKIX302.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].仅用于科研。不用于诊断过程。

1. Journal for immunotherapy of cancer

Radiotherapy enhances natural killer cell cytotoxicity and localization in pre-clinical canine sarcomas and first-in-dog clinical trial.

"25-5040 was used in Flow cytometry/Cell sorting to assess radiotherapy (RT) plus local autologous natural killer (NK) transfer in canine sarcomas."

AuthorsCanter RJ,Grossenbacher SK,Foltz JA,Sturgill IR,Park JS,Luna JI,Kent MS,Culp WTN,Chen M,Modiano JF,Monjazeb AM,Lee DA,Murphy WJ2. Veterinary immunology and immunopathologyCanine CD4+CD8+ double positive T cells in peripheral blood have features of activated T cells.AuthorsBismarck D,Schütze N,Moore P,Büttner M,Alber G,Buttlar Hv

技术参数

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