IL-4 Monoclonal Antibody (8D4-8), PE, eBioscience™

货号：12-7049-42,12-7049-41

规格：100tests,25tests

价格：2431,1038

产品类型：流式抗体

品牌：Thermo Fisher

抗原：IL-4

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号： 8D4-8

荧光染料：PE

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test（Flow）

产品详细信息Description: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.Applications Reported: The 8D4-8 antibody has been reported for use as a capture antibody for human IL-4 ELISA and for intracellular staining for flow cytometric analysis.Applications Tested: This 8D4-8 antibody is offered in 2 formats:- µg size: has been tested by intracellular staining for flow cytometric analysis. This can be used at less than or equal to 0.5 µgper test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining for flow cytometric analysis. This can be used at test size: 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.仅用于科研。不用于诊断过程。

数据

IL-4 Antibody (12-7049-42) in FlowNormal human peripheral blood cells were stimulated with PMA and Ionomycin in the presence of brefeldin A for 6 hours. The cells were surface stained with Anti-Human CD3 FITC (Product # 11-0038-42) and intracellularly stained with Anti-Human IL-4 PE (right panel). Left panel demonstrates unlabelled antibody blocking control.

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参考文献：

1. Clinical orthopaedics and related researchDo patients with a failed metal-on-metal hip implant with a pseudotumor present differences in their peripheral blood lymphocyte subpopulations?"12-7049 was used in Flow cytometry/Cell sorting to reveal the presence of a type IV hypersensitivity reaction in patients with a failed hip implant with a pseudotumour."AuthorsCatelas I,Lehoux EA,Hurda I,Baskey SJ,Gala L,Foster R,Kim PR,Beaulé PEYear2015SpeciesHuman 2. ChestT-helper 17 cell polarization in pulmonary arterial hypertension."12-7049 was used in Flow cytometry/Cell sorting to study T helper 17 cell immune polarization in patients with pulmonary arterial hypertension."AuthorsHautefort A,Girerd B,Montani D,Cohen-Kaminsky S,Price L,Lambrecht BN,Humbert M,Perros FYear2015SpeciesHuman 3. Immunity & ageing : I & ADepressive symptoms post hip fracture in older adults are associated with phenotypic and functional alterations in T cells."12-7049 was used in Flow cytometry/Cell sorting to investigate the synergistic effects of hip fracture physical trauma and psychological depressive symptoms on the aged immune system."AuthorsDuggal NA,Upton J,Phillips AC,Hampson P,Lord JMYear2015SpeciesHuman 4. The Journal of experimental medicineBET bromodomain inhibition suppresses TH17-mediated pathology."12-7049 was used in Flow cytometry/Cell sorting to identify BET proteins as a fundamental link between chromatin signalling and T(H)17 biology."AuthorsMele DA,Salmeron A,Ghosh S,Huang HR,Bryant BM,Lora JMYear2013SpeciesHuman 5. Journal of immunological methodsBrefeldin A, but not monensin, enables flow cytometric detection of interleukin-4 within peripheral T cells responding to ex vivo stimulation with Chlamydia trachomatis."12-7049 was used in Flow cytometry/Cell sorting to compare the ability of brefeldin A and monensin to concurrently trap 4 cytokines within peripheral blood mononuclear cells."AuthorsVicetti Miguel RD,Maryak SA,Cherpes TL

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