CD11c Monoclonal Antibody (N418), PE-Cyanine7, eBioscience™

货号：25-0114-82,25-0114-81

规格：100 µg,50 µg

价格：3050,1897

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD11c

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号： N418

荧光染料：PE-Cy7

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience™
浓度：	0.2 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.Applications Reported: This N418 antibody has been reported for use in flow cytometric analysis.Applications Tested: This N418 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes. CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.仅用于科研。不用于诊断过程。

数据

CD11c Antibody (25-0114-82) in FlowStaining of mouse splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Armenian Hamster IgG Isotype Control PE-Cyanine7 (Product # 25-4888-82) (left) or 0.25 µg of Anti-Mouse CD11c PE-Cyanine7 (right). Total viable cells were used for analysis.

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参考文献：

1. Frontiers in cellular neuroscienceRole of the Fractalkine Receptor in CNS Autoimmune Inflammation: New Approach Utilizing a Mouse Model Expressing the Human CX3CR1I249/M280Variant."25-0114 was used in Flow cytometry/Cell sorting to provide validation of defective function of the hCX3CR1I249/M280 variant and the foundation to broaden the understanding of microglia dysfunction during neuroinflammation."AuthorsCardona SM,Kim SV,Church KA,Torres VO,Cleary IA,Mendiola AS,Saville SP,Watowich SS,Parker-Thornburg J,Soto-Ospina A,Araque P,Ransohoff RM,Cardona AEYear2020SpeciesMouse 2. Frontiers in immunologyA β2-Integrin/MRTF-A/SRF Pathway Regulates Dendritic Cell Gene Expression, Adhesion, and Traction Force Generation."25-0114 was used in Flow cytometry/Cell sorting to demonstrate that mutation of the kindlin-3 binding site in the β2-integrin abolishes activation of the actin-regulated myocardin related transcription factor A/serum response factor signalling pathway in dendritic cells and MRTF-A/SRF-dependent gene expression."AuthorsGuenther C,Faisal I,Uotila LM,Asens ML,Harjunpää H,Savinko T,Öhman T,Yao S,Moser M,Morris SW,Tojkander S,Fagerholm SCYear2020SpeciesMouse 3. Nature communicationsNegative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression."25-0114 was used in Flow cytometry/Cell sorting to study the contribution of post-initiation steps to macrophage activation."AuthorsYu L,Zhang B,Deochand D,Sacta MA,Coppo M,Shang Y,Guo Z,Zeng X,Rollins DA,Tharmalingam B,Li R,Chinenov Y,Rogatsky I,Hu XYear2020SpeciesMouseDilution1:400 4. Nature communicationsDiversity in medullary thymic epithelial cells controls the activity and availability of iNKT cells."25-0114 was used in Flow cytometry/Cell sorting to study the mechanism that controls the development of αβ T cell lineages in the thymus."AuthorsLucas B,White AJ,Cosway EJ,Parnell SM,James KD,Jones ND,Ohigashi I,Takahama Y,Jenkinson WE,Anderson GYear2020SpeciesMouseDilution1:800 5. CellsL-Selectin/CD62L is a Key Driver of Non-Alcoholic Steatohepatitis in Mice and Men."25-0114 was used in Flow cytometry/Cell sorting to investigate the functional role of CD62L in NASH in humans as well as in two mouse models of steatohepatitis."AuthorsDrescher HK,Schippers A,Rosenhain S,Gremse F,Bongiovanni L,Bruin A,Eswaran S,Gallage SU,Pfister D,Szydlowska M,Heikenwalder M,Weiskirchen S,Wagner N,Trautwein C,Weiskirchen R,Kroy D

技术参数

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