CD1d Monoclonal Antibody (1B1), PE, eBioscience™

货号：12-0011-81,12-0011-82,12-0011-83

规格：50 µg,100 µg,200 µg

价格：1132,1959,3050

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD1d

物种：小鼠

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：1B1

荧光染料：PE

类型:	一抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™
浓度：	0.2 mg/mL	用法：	0.125 µg/test（Flow）

产品详细信息Description: The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural homology to MHC class I molecules. While similar to MHC Class I, CD1d associates with beta2m, functionally CD1d is similar to MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes. 1B1 detects beta2m associated CD1d.Applications Reported: The 1B1 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 1B1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD1d is a 49 kDa heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins. The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.仅用于科研。不用于诊断过程

数据

CD1d Antibody (12-0011-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2b K Isotype Control PE (Product # 12-4031-82) (left) or 0.06 µg of Anti-Mouse CD1d PE (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Cancer immunology, immunotherapy : CIIHistone deacetylase inhibitors enhance CD1d-dependent NKT cell responses to lymphoma."12-0011 was used in Flow cytometry/Cell sorting to demonstrate the efficacy of HDACi in restoring anti-tumor responses to mantle cell lymphoma through both cell-intrinsic and cell-extrinsic mechanisms."AuthorsTiper IV,Webb TJYear2016SpeciesMouse 2. OncoimmunologyCXCL16-positive dendritic cells enhance invariant natural killer T cell-dependent IFNγ production and tumor control."12-0011 was used in Flow cytometry/Cell sorting to study the co-stimulatory role of CXCR6/CXCL16 interactions in glycolipid-dependent invariant natural killer T (iNKT) cell activation and tumour control, showing that CXCL16-positive dendritic cells enhance iNKT cell-dependent IFNγ production and tumor control."AuthorsVeinotte L,Gebremeskel S,Johnston BYear2016SpeciesMouse 3. Proceedings of the National Academy of Sciences of the United States of AmericaTissue-specific DNA demethylation is required for proper B-cell differentiation and function."12-0011 was used in Flow cytometry/Cell sorting to determine the effect of DNA demethylation at specific sites on B cell differentiation and function."AuthorsOrlanski S,Labi V,Reizel Y,Spiro A,Lichtenstein M,Levin-Klein R,Koralov SB,Skversky Y,Rajewsky K,Cedar H,Bergman YYear2016SpeciesMouse 4. International journal of clinical and experimental medicineObesity enhances Th2 inflammatory response via natural killer T cells in a murine model of allergic asthma."12-0011 was used in Flow cytometry/Cell sorting to investigate the mechanisms connecting the development of asthma and obesity."AuthorsChen YP,Zhang JH,Li CQ,Sun QX,Jiang XHYear2015SpeciesMouse 5. The Journal of experimental medicineBAFF activation of the ERK5 MAP kinase pathway regulates B cell survival."12-0011 was used in Flow cytometry/Cell sorting to reveal a potentially critical role for the MEK5-ERK5 MAP kinase signalling pathway in BAFF-induced mature B cell survival and homeostatic maintenance of B2 cell numbers."AuthorsJacque E,Schweighoffer E,Tybulewicz VL,Ley SC

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