iNOS Monoclonal Antibody (CXNFT), PE, eBioscience™

货号：12-5920-82,12-5920-80

规格：100 µg,25 µg

价格：3163,1267

产品类型：流式抗体

品牌：Thermo Fisher

抗原：iNOS

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号： CXNFT

荧光染料：PE

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™
浓度：	0.2 mg/mL	用法：	0.06 µg/test（Flow）

产品详细信息Description: This CXNFT monoclonal antibody reacts to mouse NOS2 (inducible NOS, iNOS). Nitric oxide synthase enzymes catalyze the formation of nitric oxide from L-arginine through an NADPH- and oxygen-dependent mechanism. There are three isoforms of NOS that are encoded by three separate genes. NOS1 (neuronal NOS, nNOS) and NOS3 (endothelial NOS, eNOS) are constitutively expressed, while NOS2 is induced in response to bacterial endotoxins and inflammatory cytokines such as IFN gamma and TNF alpha. NOS2 is expressed by myeloid-derived suppressor cells and M1 macrophages but not alternatively activated M2 macrophages. NOS enzymes are functionally active only when they form homodimers, and dimerization of NOS2 occurs at steady-state concentrations of free Ca2+ such that NOS2 is functionally active when it is produced.Applications Reported: This CXNFT antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This CXNFT antibody has been tested by intracellular staining and flow cytometric analysis of LPS-stimulated mouse thioglycolate-elicited peritoneal exudate cells using the intracellular Fixation and Permeabilization Buffer Set (cat. 88-8824) and protocol. The Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) may also be used with similar results. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.仅用于科研。不用于诊断过程。

数据

iNOS Antibody (12-5920-82) in FlowMouse thioglycolate-elicited peritoneal exudate cells were stimulated overnight with LPS then surface stained with Anti-Mouse CD11b PerCP-Cyanine5-5 (Product # 45-0112-82) followed by fixation and permeabilization with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). The cells were then intracellularly stained with 0.03 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.03 µg of Anti-Mouse NOS2 PE (right). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献：

1. Nature communicationsChloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype.AuthorsChen D,Xie J,Fiskesund R,Dong W,Liang X,Lv J,Jin X,Liu J,Mo S,Zhang T,Cheng F,Zhou Y,Zhang H,Tang K,Ma J,Liu Y,Huang BYear2018SpeciesMouse 2. BMC complementary and alternative medicineOral administration of taheebo (Tabebuia avellanedae Lorentz ex Griseb.) water extract prevents DSS-induced colitis in mice by up-regulating type II T helper immune responses."12-5920 was used in Flow cytometry/Cell sorting to show that taheebo water extract (TWE) is an excellent natural product with therapeutic effects to help improve inflammatory disorders such as colitis."AuthorsPark HJ,Lee SW,Kwon DJ,Heo SI,Park SH,Kim SY,Hong SYear2017SpeciesMouse 3. Nature communicationsNatural variation of macrophage activation as disease-relevant phenotype predictive of inflammation and cancer survival."12-5920 was used in Flow cytometry/Cell sorting to highlight natural activation diversity as a disease relevant dimension in macrophage biology."AuthorsBuscher K,Ehinger E,Gupta P,Pramod AB,Wolf D,Tweet G,Pan C,Mills CD,Lusis AJ,Ley KYear2017SpeciesMouse 4. Immunity, inflammation and diseaseLocation of tumor affects local and distant immune cell type and number."12-5920 was used in Flow cytometry/Cell sorting to demonstrate that location of tumour, and not simply the tumour itself, has a definitive role in regulating immune effectors."AuthorsHensel JA,Khattar V,Ashton R,Lee C,Siegal GP,Ponnazhagan SYear2017SpeciesMouse 5. Scientific reportsModulation of Microglial Cell Fcγ Receptor Expression Following Viral Brain Infection."12-5920 was used in Flow cytometry/Cell sorting to demonstrate that neuro-inflammation following viral infection increases expression of activating FcγRs on M1-polarized microglia."AuthorsChauhan P,Hu S,Sheng WS,Prasad S,Lokensgard JR

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