FOXP3 Monoclonal Antibody (FJK-16s), PE-Cyanine7, eBioscience™

货号：25-5773-82,25-5773-80

规格：100 µg,25µg

价格：4738,3257

产品类型：流式抗体

品牌：Thermo Fisher

抗原：FOXP3

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号： FJK-16s

荧光染料：PE-Cy7

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™
浓度：	0.2 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.Please note that FJK-16s has been optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (cat. 00-5523). The use of other fixation and staining buffers is not recommended.Applications Reported: This FJK-16s antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This FJK-16s antibody has been tested by intranuclear staining and flow cytometric analysis using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.仅用于科研。不用于诊断过程。

数据

FOXP3 Antibody (25-5773-82) in FlowSurface staining of BALB/c splenocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) (left) and Anti-Mouse CD25 APC (Product # 17-0251-82) (right), followed by intracellular staining with 0.25 µg of Anti-Mouse/Rat Foxp3 PE-Cyanine7 using the Foxp3 Staining Buffer Set (Product # 00-5523-00). Total viable cells were used for analysis.

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献：

1. BMC complementary and alternative medicineThe effects of freeze-dried Ganoderma lucidum mycelia on a recurrent oral ulceration rat model."25-5773 was used in Flow cytometry/Cell sorting to show that freeze-dried powder from G. lucidum mycelia could potentially be used to treat recurrent oral ulceration."AuthorsXie L,Zhong X,Liu D,Liu L,Xia ZYear2017SpeciesRat 2. ImmunologyThe NLRP3 inflammasome contributes to host protection during Sporothrix schenckii infection."25-5773 was used in Flow cytometry/Cell sorting to indicate that the NLRP3 inflammasome contributes to protecion against infection from S. schenckii by linking the innate recognition of S. schenckii to the adaptive immune response."AuthorsGonçalves AC,Ferreira LS,Manente FA,de Faria CMQG,Polesi MC,de Andrade CR,Zamboni DS,Carlos IZYear2017SpeciesMouse 3. Stem cell research & therapyCulture supernatant of adipose stem cells can ameliorate allergic airway inflammation via recruitment of CD4+CD25+Foxp3 T cells."25-5773 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which adipose tissue stem cells (ASCs) reduce allergic symptoms and eosinophilic inflammation following injection, showing that culture supernatant of ASCs also reduced allergic airway inflammation."AuthorsYu HS,Park MK,Kang SA,Cho KS,Mun SJ,Roh HJYear2017SpeciesMouse 4. Aging cellAlternative rapamycin treatment regimens mitigate the impact of rapamycin on glucose homeostasis and the immune system."25-5773 was used in Flow cytometry/Cell sorting to identify an intermittent rapamycin dosing schedule with minimal serious side effects."AuthorsArriola Apelo SI,Neuman JC,Baar EL,Syed FA,Cummings NE,Brar HK,Pumper CP,Kimple ME,Lamming DW

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