CD4 Monoclonal Antibody (OX35), APC, eBioscience™

货号：17-0040-82,17-0040-80

规格：100 µg,25 µg

价格：2677,925

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD4

物种：大鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：OX35

荧光染料：APC

类型:	一抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The OX35 monoclonal antibody reacts with rat CD4. CD4 is a co-receptor which participates in signaling through the T cell receptor by making contacts with MHC class II expressed on antigen-presenting cells (APC). CD4 is expressed in the thymus by CD4+CD8+ "double-positive" and CD4+ "single-positive" thymocytes, and by CD4+ "helper" T cells in the periphery. CD4 is a cell-surface membrane glycoprotein which contains four extracellular immunoglobulin-like domains.ApplicationsReported: This OX35 antibody has been reported for use in flow cytometric analysis.Applications Tested: This OX35 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].仅用于科研。不用于诊断过程。

数据

CD4 Antibody (17-0040-82) in FlowStaining of rat splenocytes with Anti-Rat CD8a PerCP-eFluor® 710 (Product # 46-0084-82) and 0.125 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or 0.125 µg of Anti-Rat CD4 APC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Mediators of inflammationExploring the Mechanism on the Medullary Visceral Zone Inhibiting the Cholinergic Anti-inflammatory Pathway Induced by Sepsis."Published figure using CD4 monoclonal antibody (Product # 17-0040-82) in Flow Cytometry"2. Experimental and therapeutic medicineCombined treatment with simvastatin and rapamycin attenuates cardiac allograft rejection through the regulation of T helper 17 and regulatory T cells."Published figure using CD4 monoclonal antibody (Product # 17-0040-82) in Flow Cytometry"3. Molecular medicine reportsPlacenta‑derived mesenchymal stem cells improve airway hyperresponsiveness and inflammation in asthmatic rats by modulating the Th17/Treg balance."Published figure using CD4 monoclonal antibody (Product # 17-0040-82) in Flow Cytometry"4. Experimental and therapeutic medicineProteomic changes of CD4+/CD25+/forkhead box p3+regulatory T cells in a 30-day rat model of sepsis survival."Published figure using CD4 monoclonal antibody (Product # 17-0040-82) in Flow5.Cytometry"Molecular medicine reportsIL-17 contributed to the neuropathic pain following peripheral nerve injury by promoting astrocyte proliferation and secretion of proinflammatory cytokines."Published figure using CD4 monoclonal antibody (Product # 17-0040-82) in Flow Cytometry"

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