Rat anti Dog CD45 antibody, clone YKIX716.13 ,Rat anti Dog CD45 antibody, clone YKIX716.13

2024-10-22

Rat anti Dog CD45 antibody, clone YKIX716.13

货号:MCA1042APC

规格:100 Tests

价格:4293

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD45

物种:dog

宿主:大鼠

抗体亚型:IgG2b

克隆号:YKIX716.13

荧光染料:Alexa Fluor 488

产品介绍
Rat anti Dog CD45 antibody, clone YKIX716.13recognizes canine CD45 also known as leukocyte common antigen lustered as Canine CD45 in the First Canine Leukocyte Antigen Workshop (CLAW). Clone YKIX 716.13: immunoprecipitates an antigen of ~180/200 kDa from Con-A blasts (Cobboldet al.1994). CD45 is expressed on all leukocytes in canine peripheral blood. Rat anti Dog CD45 antibody, clone YKIX716.13 reacts with CD45 on all outbred mongrels and beagles tested and may be against CD45RB isoform.
产品详情
Target SpeciesDog
Product FormPurified IgG conjugated to Allophycocyanin (APC) - lyophilized
ReconstitutionReconstitute with 1ml distilled water
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09%Sodium Azide1%Bovine Serum Albumin
ImmunogenCanine thymocytes.
Approx. Protein ConcentrationsIgG concentration 0.05 mg/ml
Fusion PartnersSpleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
APC650661
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
产品推荐
DescriptionProduct CodeApplicationsPack Size
Rat IgG2b Negative Control:APCMCA6006APCF100 Tests
文献
1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.2.Gorman, S.D.et al.(1994) Isolation and expression of cDNA encoding the canine CD4 and CD8 alpha antigens.Tissue Antigens. 43 (3): 184-8.3.Watson, C.J.et al.(1993) CD4 and CD8 monoclonal antibody therapy: strategies to prolong renal allograft survival in the dog.Br J Surg. 80 (11): 1389-92.

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