Rat anti Dog CD45 antibody, clone YKIX716.13 Thermo ,Rat anti Dog CD45 antibody, clone YKIX716.13

2024-10-22

Rat anti Dog CD45 antibody, clone YKIX716.13

货号:MCA1042B

规格:0.1 mg

价格:3681

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD45

物种:dog

宿主:大鼠

抗体亚型:IgG2b

克隆号:YKIX716.13

荧光染料:Biotin

产品介绍
Rat anti Dog CD8 antibody, clone YCATE55.9was clustered as Canine CD8 in the First Canine Leukocyte Antigen Workshop (Cobboldet al.1994). YCATE55.9 reacts with a rat cell line transfected with cDNA for canine CD8α (Gormanet al.1994) and blocks MHC class I dependant T-cell responsesin vitroandin vivo.Rat anti Dog CD8, clone YCATE55.9 has been shown to deplete circulating CD8+ T cells when administered to dogsin vivo. (Watsonet al.1993) Reduced levels of circulating CD8+ T cells has been associated with decreased survival times for dogs with osteosarcoma (Billeret al.2010).
产品详情
Target SpeciesDog
Product FormPurified IgG conjugated to Pacific Blue ® - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
ImmunogenCanine thymocytes.
Approx. Protein ConcentrationsIgG concentration 0.05 mg/ml
Fusion PartnersSpleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
Pacific Blue®410455
AcknowledgementsThis product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
产品推荐
DescriptionProduct CodeApplicationsPack Size
Rat IgG2a Negative Control:Pacific Blue®MCA6005PBF100 Tests/1ml
文献
1.Cobbold, S/ & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.

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