Bio-Rad MCA1037PE CD5抗体,Rat anti Dog CD5: PE-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1037PE CD5抗体,Rat anti Dog CD5: PE

2024-10-22

Rat anti Dog CD5: PE

货号：MCA1037PE

规格：100 Tests/1ml

价格：4293

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD5

物种：dog

宿主：大鼠

抗体亚型：IgG2a

克隆号：YKIX322.3

荧光染料：PE

产品介绍
Rat anti Dog CD5 antibody, clone YKIX322.3recognizes canine CD5, a 67 kDa cell surface type 1 transmembrane glycoprotein also known as lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T-cells and thymocytes, CD5 is also expressed by NK cells at low levels (Huanget al.2008). Rat anti dog CD5, cloneYKIX322.3 was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop (Cobboldet al.1994).In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B-cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression (Vernau and Moore 1999). Rat anti dog CD5 serves as a useful marker for the discrimination of canine leukemias of differing origins (Deraviet al.2017).
产品详情
Target Species	Dog
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution	Reconstitute with 1.0 ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Immunogen	Concanavilin A activated canine peripheral blood cells
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Fusion Partners	Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
产品推荐
Description	Product Code	Applications	Pack Size
Rat IgG2a Negative Control:RPE	MCA1212PE	F	100 Tests
文献
1.Cobbold, S/ & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.2.Hewicker-Trautwein, M.et al.(1999) Immunocytochemical demonstration of lymphocyte subsets and MHC class II antigen expression in synovial membranes from dogs with rheumatoid arthritis and degenerative joint disease.Vet Immunol Immunopathol. 67 (4): 341-57.3.Huang, Y.C.<i.et al.<="" i="" style="box-sizing: border-box;">(2008) CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.J Leukoc Biol. 84: 1501-10.4.Araújo, M.S.et al.(2011) Immunological changes in canine peripheral blood leukocytes triggered by immunization with first or second generation vaccines against canine visceral leishmaniasis.Vet Immunol Immunopathol. 141: 64-75.

技术参数

产品应用 Flow

产品类型单克隆抗体

推荐用于 Flow

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Bio-Rad MCA1037PE CD5抗体,Rat anti Dog CD5: PE

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