| 产品介绍 | | |
| Rat anti Dog CD44 antibody, clone YKIX337.8.7recognizes canine CD44, also known as H-CAM, a single-pass type 1 membrane of approximately 90 kDa expressed by most leucocytes and epithelial cells. CD44 expression is markedly increased upon cell activation (Alldingeret al.2000).CD44 is involved in cell-cell, cell adhesion and cell migration and is the principal cellular receptor for hyaluronate via it'sLINKdomain, additionally CD44 interacts with other ligands including collagens and metalloproteinases.Altered CD44 expression is detected in many forms of invasive and metastatic cancer, CD44 expression has been observed on canine mammary and melanocytic tumors (Serraet al.2004). |
| 产品详情 | | |
| Target Species | Dog |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Raccoon | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to Alexa Fluor 488 - liquid |
| Reconstitution | Reconstitute with 1.0 ml distilled water |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Immunogen | Concanavilin A activated Canine T cells. |
| Approx. Protein Concentrations | IgG concentration 0.05mg/ml |
| Fusion Partners | Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3.myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®488 | 495 | 519 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/10 | 1/20 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood. |
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| 文献 |
| 1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54. |
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