Rat anti Dog CD4 antibody, clone YKIX302.9,Rat anti Dog CD4 antibody, clone YKIX302.9-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti Dog CD4 antibody, clone YKIX302.9,Rat anti Dog CD4 antibody, clone YKIX302.9

2024-10-22

Rat anti Dog CD4 antibody, clone YKIX302.9

货号：MCA1038PECY7

规格：100 Tests/1ml

价格：4395

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD4

物种：dog

宿主：大鼠

抗体亚型：IgG1

克隆号：YKIX302.9

荧光染料：PE-Cy7

产品介绍
Rat anti Dog CD4 antibody, clone YKIX302.9, is a monoclonal antibody specific for the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobboldet al.1992] along with cloneCA13.1E4.Rat anti Dog CD4 antibody, clone YKIX302.9 partially depletes circulating T lymphocytes when administeredin vivo, but alone is not sufficient to prolong allograft survival in a canine transplant model (Watsonet al.1993).Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocyte subsets (Mooreet al.1992).
产品详情
Target Species	Dog
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE)-Cy7 - lyophilized
Reconstitution	Reconstitute with 1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Immunogen	Concanavilin A activated canine peripheral blood cells
Approx. Protein Concentrations	IgG concentration 0.05mg/ml
Fusion Partners	Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE-Cy7 488nm laser	496	695
Acknowledgements	Cy® and CyDye® are registered trademarks of GE Healthcare
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
产品推荐
Description	Product Code	Applications	Pack Size
Rat IgG2a Negative Control:RPE-Cy7	MCA6005PECY7	F	100 Tests/1ml
文献
1.Cobbold, S. & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.

技术参数

产品应用 Flow

产品类型单克隆抗体

推荐用于 Flow

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Rat anti Dog CD4 antibody, clone YKIX302.9,Rat anti Dog CD4 antibody, clone YKIX302.9

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