Rat anti Dog CD4 antibody, clone YKIX302.9 MCA1038GA bio-rad,Rat anti Dog CD4 antibody, clone YKIX302.9

2024-10-22

Rat anti Dog CD4 antibody, clone YKIX302.9

货号:MCA1038GA

规格:0.1 mg

价格:2151

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD4

物种:dog

宿主:大鼠

抗体亚型:IgG1, kappa

克隆号:YKIX302.9

荧光染料:Purified

产品介绍
Rat anti Dog CD4 antibody, clone YKIX302.9, is a monoclonal antibody specific for the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobboldet al.1992] along with cloneCA13.1E4.Rat anti Dog CD4 antibody, clone YKIX302.9 partially depletes circulating T lymphocytes when administeredin vivo, but alone is not sufficient to prolong allograft survival in a canine transplant model (Watsonet al.1993).Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocyte subsets (Mooreet al.1992).
产品详情
Target SpeciesDog
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)
ImmunogenConcanavilin A activated canine peripheral blood cells
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
Fusion PartnersSpleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/501/100
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
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文献
1.Cobbold, S/ & Metcalfe, S. (1994) Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW).Tissue Antigens. 43 (3): 137-54.

技术参数

产品应用 Flow

产品类型 单克隆抗体

推荐用于 Flow

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