KDM6A Polyclonal Antibody

货号：PA5-31828

规格：100 µL

价格：5712

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant fragment corresponding to a region wit

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1.36 mg/mL	用法：	1:100-1:1000（ICC）；1:100-1:1000（IF）；1:100-1:1000（IHC(P)）；1:100-1:500（IP）；1:500-1:3000(WB)

产品详细信息Recommended positive controls: SK-N-SH, IMR32, SK-N-AS.Predicted reactivity: Mouse (93%), Xenopus laevis (82%), Dog (86%), Bovine (98%).Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3.

数据：

KDM6A Antibody (PA5-31828) in IFImmunofluorescent analysis of KDM6A in paraformaldehyde-fixed MCF-7 cells using a KDM6A polyclonal antibody (Product # PA5-31828) at a 1:500 dilution. KDM6A Antibody (PA5-31828) in IHC (P)Immunohistochemistry (Paraffin) analysis of KDM6A was performed in paraffin-embedded mouse spleen tissue using KDM6A Polyclonal Antibody (Product # PA5-31828) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min. KDM6A Antibody (PA5-31828) in WBWestern blot analysis of UTX/KDM6A was performed by loading 2 µg of molecular weight marker (MWM), 40 µg of ARD (a KDM6A negative cell line) or 40 µg of ARP-1 (a matched KDM6A positive cell line) cell lysate onto a NuPAGE 4-12% Bis-Tris Gel (Product # NP0321BOX) and running with Novex NuPAGE MES SDS Running buffer (Product # NP0002). Proteins were transferred to Immobulon-FL PVDF membrane and blocked for 1 hour in LI-COR blocking buffer. Membrane was incubated overnight at 4°C in LI-COR blocking buffer containing a 1:1000 dilution of KDM6A polyclonal antibody (Product # PA5-31828), followed by a 1 hour room temperature incubation in TBST containing a 1:10000 dilution of 680 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 680 (Product # A32734). Detection of KDM6A at approximately 150 kDa (indicated by the arrow) was performed using a LI-COR Odyssey CLx using the 700nm channel. (left) red channel image (right) inverted grey scale image. Data courtesy of Dr. Jonathan D. Licht's laboratory.

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