Magic Red™ Cathepsin B Kit ICT938 BIO-RAD ,Magic Red™ Cathepsin B Kit

2024-10-22

Magic Red™ Cathepsin B Kit

货号:ICT938,ICT937

规格:100 Tests,25 Tests

价格:6146,2763

产品类型:细胞增殖活性检测

品牌:Bio-Rad

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:其它

产品介绍
The Magic Red Cathepsin B kit uses a quick and easy method to analyze intracellular Cathepsin B protease activity in whole living cells.
产品详情
Product FormPack Size: 25 TestsMagic Red Substrate (MR-RR2), 25 Tests - lyophilizedHoechst 33342, 1 mLAcridine Orange, 0.5 mLPack Size: 100 TestsMagic Red Substrate MR-(RR)2, 100 Tests - lyophilizedHoechst 33342, 1 mLAcridine Orange, 0.5 mL
Test PrincipleCathepsin Magic Red kits measure cathepsin B protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.The Magic Red reagent contains a cathepsin B target sequence peptide (RR)2linked to a red (Cresyl Violet) fluorescent probe.The Cathepsin Magic Red kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
Approx. Protein ConcentrationsMagic Red™ is a trademark of Immunochemistry Technologies, LLC.
RegulatoryFor research purposes only
GuaranteeGuaranteed until date of expiry. Please see product label.
存储条件
Store the unopened kit and each unopened component at +4℃ until the expiration date.Once reconstituted with DMSO, use Magic Red reagent immediately, or store at -20℃ for 6 months protected from light and thawed no more than twice during that time.
应用
Application NameVerifiedMin DilutionMax Dilution
Immunofluorescence
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
产品推荐
DescriptionProduct CodeApplicationsPack Size
Magic Red™ Cathepsin B KitICT938IF100 Tests
文献
1.Hämälistö, S.et al.(2020) Spatially and temporally defined lysosomal leakage facilitates mitotic chromosome segregation.Nat Commun. 11 (1): 229.

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产品类型 细胞活性检测

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