Magic Red™ Caspase-3/7 Kit ICT936 BIO-RAD ,Magic Red™ Caspase-3/7 Kit

2024-10-22

Magic Red™ Caspase-3/7 Kit

货号:ICT936,ICT935

规格:100 Tests,25 Tests

价格:6103,2763

产品类型:细胞凋亡检测

品牌:Bio-Rad

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:其它

产品介绍
The Magic Red Caspase-3/7 kituses a quick and easy method to analyze intracellular active caspases in apoptotic cells.
产品详情
Product FormPack Size: 25 TestsMagic Red Substrate MR-(DEVD)2, 25 Tests - lyophilizedHoechst 33342, 1 mLAcridine Orange, 0.5 mLPack Size: 100 TestsMagic Red Substrate MR-(DEVD)2, 100 Tests - lyophilizedHoechst 33342, 1 mLAcridine Orange, 0.5 mL
Test PrincipleCaspase Magic Red kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of caspases to produce a fluorescent product. The Magic Red reagent contains a caspase inhibitor sequence (DEVD)2linked to a red (Cresyl Violet) fluorescent probe.The Caspase Magic Red kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
Approx. Protein ConcentrationsMagic Red™ is a trademark of Immunochemistry Technologies, LLC.
RegulatoryFor research purposes only
GuaranteeGuaranteed until date of expiry. Please see product label.
存储条件
Store the unopened kit and each unopened component at +4℃ until the expiration date.Once reconstituted with DMSO, use Magic Red reagent immediately, or store at -20℃ for 6 months protected from light and thawed no more than twice during that time.
应用
Application NameVerifiedMin DilutionMax Dilution
Immunofluorescence
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
产品推荐
DescriptionProduct CodeApplicationsPack Size
Magic Red™ Caspase-3/7 KitICT936IF100 Tests
文献
1.Wei, Z.et al.(2011) Notch activation enhances the microglia-mediated inflammatory response associated with focal cerebral ischemia.Stroke. 42 (9): 2589-94.2.Shibata, Y.et al.(2016) Controlled in-cell release of caspase from photodegradable nanoparticles using the PARCEL methodApplied Spectroscopy Reviews. Mar 13 [Epub ahead of print]3.McHugh, B.J.et al.(2019) Cathelicidin is a "fire alarm", generating protective NLRP3-dependent airway epithelial cell inflammatory responses during infection withPseudomonas aeruginosa..PLoS Pathog. 15 (4): e1007694.

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产品应用 IF

产品类型 细胞凋亡检测

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