Green Cathepsin B Kit BIO-RAD ICT9151,Green Cathepsin B Kit-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Green Cathepsin B Kit BIO-RAD ICT9151,Green Cathepsin B Kit

2024-10-22

Green Cathepsin B Kit

货号：ICT9151

规格：25 Tests

价格：3970

产品类型：检测试剂盒

品牌：Bio-Rad

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

产品介绍
Green Cathepsin B Kitenables the quantitation and monitoring of intracellular cathepsin activity over time in vitro. The Rhodamine 110 Cathepsin B substrate reagent is a non-cytotoxic and membrane permeant substrate that fluoresces green upon cleavage by active cathepsin enzymes.
产品详情
Reagents in the Kit	1 vial of Rhodamine110-(RR)2substrate - lyophilized10X Cellular Assay Buffer, 15 mlHoechst Stain, 1 ml
Test Principle	Rhodamine 110 Cathepsin B substrate utilizes the photostable green fluorophore, rhodamine 110. Rhodamine 110 cathepsin B substrate is comprised of rhodamine 110 coupled to two copies of the amino acid sequence, arginine-arginine (RR), which is the preferential target sequence for cathepsin B. When bi-substituted via amide linkage to two cathepsin B target peptide sequences, rhodamine110 is nonfluorescent. Following enzymatic cleavage at one or both arginine (R) amide linkage sites, the mono and non-substituted rhodamine 110 fluorophores generate green fluorescence when excited at 500 nm.To use the Green Cathepsin B Assay, simply add the Rhodamine 110 Cathepsin B substrate [R110-(RR)2] directly to the cell culture media (or 1X Cellular Assay Buffer), incubate, and analyze. Because R110-(RR)2 is cell-permeant, it easily penetrates the cell membrane and the membranes of the internal cellular organelles - no lysis or permeabilization steps are required. R110-(RR)2 will enter the cell in a non-fluorescent state. If cathepsin enzymes are active, they will cleave off the two arginine-arginine cathepsin B targeting sequences and allow the rhodamine 110 fluorophore to become fluorescent upon excitation. By varying the duration and concentration of exposure to the R110-(RR)2 substrate, a picture can be obtained of the relative abundance and intracellular location of cathepsin enzymatic activity. Positive cells will fluoresce green, while negative cells will exhibit very low levels of background green fluorescence. There is no interference from pro-cathepsins forms of the enzymes. If the treatment or experimental condition stimulates cathepsin activity, cells containing elevated levels of cathepsin activity will appear brighter green than cells with lower levels of cathepsin activity.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Rhodamine110-(RR)2	525	535
Regulatory	For research purposes only
Guarantee	Guaranteed until date of expiry. Please see product label.
存储条件
MULTIPLE STORAGE CONDITIONS APPLY ON ARRIVAL. Store the unopened kit (and each unopened component) according to the storage instructions on each component label. Store the Rhodamine110-(RR)2substrate at -20℃. Once reconstituted in DMSO, use the Rhodamine110-(RR)2substrate immediately or aliquot and store at -20℃ for 6 months, protected from light. Avoid repeated freezing and thawing
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Refer to Instructions for Use
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 FLOW;IF

推荐用于 FLOW;IF

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-41352.htm

特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系删除，本平台不提供信息存储服务。

Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.

产品询价需求提交

Green Cathepsin B Kit BIO-RAD ICT9151,Green Cathepsin B Kit

相关推荐