Recombinant Human IgG4 kappa, AbD18705_hIgG4 HCA195 BIO-RAD,Recombinant Human IgG4 kappa, AbD18705_hIgG4

2024-10-22

Recombinant Human IgG4 kappa, AbD18705_hIgG4

货号:HCA195,HCA195G

规格:0.1 mg,1 mg

价格:1972,17340

产品类型:荧光二抗

品牌:Bio-Rad

物种:人

宿主:其它

抗体亚型:IgG4 Kappa

克隆号:AbD18705_hIgG4

荧光染料:Purified

产品介绍
Recombinant Human IgG4 kappa, AbD18705_hIgG4is a recombinant human IgG4 antibody with a kappa light chain.AbD18705_hIgG4 is specific for green fluorescent protein (GFP) and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgG4 levels, or as a control antibody when using other human antibodies of the same isotype and subclass.In addition to the tetrameric IgG structure, composed of two copies each of the heavy and light chains, human IgG4 forms so-called half-molecules. This species contains only one heavy and one light chain. Half-molecules can assemble with an unrelated half-molecule to form bispecific tetrameric antibody species (Aalberseet al.2002). Half-molecules of human IgG4 preparations can be detected as an additional species under denaturing, non-reducing conditions using SDS-PAGE, size exclusion chromatography or capillary electrophoresis.Mutation in the core hinge region of IgG4 prevents the formation of half-molecules (Bloomet al.1997). In addition to the wild type recombinant, Bio-Rad also offers recombinant Human IgG4 kappa in its mutated form (HCA247). Recombinant Human IgG4 lambda is also available in both wild type (HCA050) and mutant form (HCA246).
产品详情
Product FormHuman IgG4 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
PreparationPurified IgG prepared by affinity chromatography on Protein A
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.01% Thiomersal
ImmunogenGreen Fluorescent Protein (GFP).
Approx. Protein ConcePurityntrationsPack Size: 0.1 mgAntibody concentration 0.5 mg/mlPack Size: 1 mgAntibody concentration 1.0 mg/ml
AcknowledgementsThis product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
产品推荐
DescriptionProduct CodeApplicationsPack Size
Recombinant Human IgG4 Lambda (Mutant)HCA246E0.1 mg

技术参数

产品应用 elisa

产品类型 重组蛋白

推荐用于 elisa

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