Human anti Human desmin, clone AbD03744 HCA023 bio-rad,Human anti Human desmin, clone AbD03744-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Human anti Human desmin, clone AbD03744 HCA023 bio-rad,Human anti Human desmin, clone AbD03744

2024-10-22

Human anti Human desmin, clone AbD03744

货号：HCA023

规格：0.1 mg

价格：2023

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：desmin

物种：人

宿主：人

抗体亚型：HuCAL Fab bivalent

克隆号：AbD03744

荧光染料：Purified

产品介绍
Human anti Human desmin, clone AbD03744recognizes desmin, a member of the class III family of intermediate filaments. Desmin constitutes part of the cytoskeleton, and is the characteristic intermediate filament of all three types of muscle cells (skeletal, cardiac and smooth muscle).Desmin is a 52 kDa protein encoded by nine exons of a gene located at 2q35. Desmin forms a cytoskeletal network across the muscle fiber bordering at the plasma and nuclear membrane and is particularly localized to the subplasmalemmal region and the Z-band. Staining for desmin is useful for identification of rhabdomyosarcomas and leiomyomas.For use in immunohistology staining we recommend the use of an anti-His tag bridging antibody, followed by visualization using the Dako Duet staining system.
产品详情
Target Species	Human
Product Form	A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed inE. Coliand purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
Preparation	Metal chelate affinity chromatography
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.01% Thiomersal
Immunogen	Recombinant human desmin produced inE. coli.
Approx. Protein ConcePurityntrations	Antibody concentration 0.5 mg/ml.
Acknowledgements	This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.His-tag is a registered trademark of EMD Biosciences.
Regulatory	For research purposes only
Guarantee	6 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√		2ug/ml
Immunohistology - Frozen	√	1/100	1/500
Western Blotting	√	1/200	1/2000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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技术参数

产品应用 elisa；wb

产品类型单克隆抗体

推荐用于 elisa；wb

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Human anti Human desmin, clone AbD03744 HCA023 bio-rad,Human anti Human desmin, clone AbD03744

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