Mouse anti Rat CD4:FITC/CD25:RPE dual color reagent DC040 ,Mouse anti Rat CD4:FITC/CD25:RPE dual color reagent

2024-10-22

Mouse anti Rat CD4:FITC/CD25:RPE dual color reagent

货号:DC040

规格:100 Tests

价格:3596

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD4/CD25

物种:大鼠

宿主:小鼠

抗体亚型:其它

克隆号:W3/25 / OX-39

荧光染料:PE

产品介绍
Mouse anti Rat CD4:FITC/CD25:RPE dual color reagentis a dual colour reagent designed to allow the simultaneous enumeration of cells expressing the rat CD4 and CD25 cell surface antigens.Clone W3/25 recognises rat CD4, a T cell differentiation antigen expressed by helper T lymphocytes and weakly by monocytes. Clone OX-39 recognises rat CD25, a ~55 kDa glycoprotein expressed by activated T lymphocytes.
产品详情
Target SpeciesRat
Product FormDual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
ReconstitutionReconstitute with 1 ml distilled water
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09%Sodium Azide (NaN3)1%Bovine serum albumin
Antibody IsotypesFITC reagent: IgG1 (MOUSE)RPE reagent: IgG1 (MOUSE)
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578
RPE 561nm laser546578
FITC490525
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.This product should be stored undiluted.DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Barclay, A. N.et al. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.Immunol. 42: 593-600.2.Paterson, D.J.et al.(1987) Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts.Mol Immunol. 24 (12): 1281-90.3.Zschemisch, N.H.et al.(2012) Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat.BMC Immunol. 13: 60.

技术参数

产品应用 flow

产品类型 Dual Color Reagent

推荐用于 flow

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