Mouse Seroblock FcR BUF041A bio-rad,Mouse Seroblock FcR

2024-10-22

Mouse Seroblock FcR

货号:BUF041A,BUF041B

规格:0.1 mg,0.5 mg

价格:1547,4658

产品类型:辅助试剂

品牌:Bio-Rad

物种:其它

宿主:小鼠

抗体亚型:IgG2b

荧光染料:其它

产品介绍
Mouse Seroblock FcRis a rat antibody that recognizes mouse CD16 and CD32, cell surface proteins also known as FcRγIII and FcRγII, respectively. The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response. CD16 and/or CD32 are expressed by a wide variety of cells, including monocytes, macrophages, B lymphocytes, granulocytes, NK cells, dendritic cells and some activated T lymphocytes. The expression of CD16/CD32 antigens can lead to non-specific binding of test monoclonal antibodies in staining procedures, resulting in “high background” staining on a wide range of cells. This non-specific binding may be blocked by pre-incubation of target cells with BUF041A, resulting in clearer staining. For direct analysis of CD16/32 expression this antibody is also available conjugated directly to FITC (MCA2305F), AlexaFluor488 (MCA2305A488), AlexaFluor647 (MCA2305A647) and RPE (MCA2305PE).
产品详情
Target SpeciesMouse
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenPU5 1.8 IOE7 Balb/c mouse cell line.
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Pack Size: 0.1 mgThis product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the product. Storage in frost-free freezers is not recommended.Pack Size: 0.5 mgThis product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Ivanovska, N.Det al. (2008) Properdin Deficiency in Murine Models of Nonseptic Shock.The Journal of Immunology. 180: 6962-6969.2.Chen, H-Fet al. (2009) A reduced oxygen tension (5%) is not beneficial for maintaining human embryonic stem cells in the undifferentiated state with short splitting intervals.Human Reproduction. 24: 71-80.3.Birjandi, S.Z.et al.(2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.J Immunol. 186: 3441-51.

技术参数

产品应用 flow

产品类型 Accessory Reagent

推荐用于 flow

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