Rabbit anti Human BMP-2 antibody AHP960B bio-rad,Rabbit anti Human BMP-2 antibody

2024-10-22

Rabbit anti Human BMP-2 antibody

货号:AHP960B

规格:50 µg

价格:3936

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:BMP-2

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human BMP-2 antibodyrecognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 2 (BMP-2/BMP-2a), full-length 396 amino acids. BMP-2 is a member of the Transforming Growth Factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.The realization that BMP-2 was involved in the stimulation of bone formation emerged from research in spinal surgery, following the discovery that the healing of bones was directed by proteins contained within the bone matrix itself.
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to Biotin - lyophilized
Buffer SolutionPhosphate buffered saline
ReconstitutionReconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.N.B. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum PreparationAntisera to human BMP-2 were raised by repeated immunisations of rabbits with highly purified antigen.
Preservative StabilisersNone present.
ImmunogenRecombinant human BMP-2
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.25 ug/ml1.0 ug/ml
Immunohistology - Paraffin10.1 ug/ml0.1 ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg
文献
1.Bessa, P.C.et al.(2008) Osteoinduction in human fat-derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli.Biotechnol Lett. 30 (1): 15-21.

技术参数

产品应用 wb

产品类型 多克隆抗体

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