Bio-Rad 0178-0149G ACTH,Mouse anti Human ACTH

2024-10-23

Mouse anti Human ACTH

货号:0178-0149G,0178-0149

规格:1 mg,0.2 mg

价格:5100,3043

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:ACTH

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:57 (BGN/1388/66)

荧光染料:Purified

产品介绍
Mouse anti Human ACTH antibody (Clone 57, BGN/1388/66)recognizes 1-24 ACTH (Synacthen) which, as a synthetic analogue of naturally-occurring Adrenocorticotropic Hormone, can be used to measure adrenal reserve and for the diagnosis of adrenal insufficiency by acute adrenocortical stimulation.ACTH, released from the anterior pituitary gland in response to corticotropin-releasing hormone from the hypothalamus, acts on the adrenal cortex to stimulate the production of corticosteroids such as cortisol, involved in the response to stress. Studies have shown that the administration of 1-24 ACTH increases blood pressure, believed to be attributed to an increase in ACTH-stimulated cortisol secretion, in association with increased cardiac output.Mouse anti Human ACTH antibodydoes not recognize 17-39 ACTH (CLIP). It does cross-reacts with rat N-terminal ACTH.
产品详情

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%Sodium Azide (NaN3)

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Immunogen

Synthetic peptide corresponding to the amino acids 1 - 24 (SYSMEHFRWGKPVGKKRRPVKVYP) of the human ACTH conjugated to Keyhole Limpet Haemocyanin.

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

Carrier Free

Yes
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

Immunofluorescence

Immunohistology - Paraffin1

1/1001/200
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.

1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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文献

References for ACTH antibody1. Zhou, Met al.(2016) Localization of ATP-sensitive K+channel subunits in rat pituitary gland.Arch Histol Cytol. 76 (1): 53-66.Further Reading2. Hatakeyama, H.et al.(2000) Functional adrenocorticotropic hormone receptor in cultured human vascular endothelial cells : possible role in control of blood pressure.Hypertension. 36 (5): 862-5.

技术参数

产品应用 0178-0149G:IHC(F);IF;IHC(P)*0178-0149:ELISA;IHC(P)*

产品类型 Monoclonal Antibody

推荐用于 0178-0149G:IHC(F);IF;IHC(P)*0178-0149:ELISA;IHC(P)*

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