Rabbit anti human EGF AHP767 bio-rad ,Rabbit anti human EGF

2024-10-22

Rabbit anti human EGF

货号:AHP767

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:EGF

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:其它

产品介绍
Rabbit anti Human EGFrecognizes human Epidermal Growth Factor, a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, expressed by a variety of cells.The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the ~170 kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a (transforming growth factor alpha), VGF (vaccinia virus growth factor) and Betacellulin, expressed in the pancreas, small intestine and by certain tumour cells (Senoet al.1996). Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.
产品详情
Target SpeciesHuman
Product FormPurified IgG - lyophilized
ImmunogenRecombinant human EGF (PHP030A).
ReconstitutionReconstitute with 0.1ml distilled water.For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add azide.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml after reconstitution
Antiserum PreparationAntisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen.Purified IgG prepared by affinity chromatography.
Preservative StabilisersNone present.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.52ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg
文献
1.Carpenter, G. & Cohen, S. (1990) Epidermal growth factor.J Biol Chem. 265 (14): 7709-12.

技术参数

产品应用 elisa;wb

产品类型 Polyclonal Antibody

推荐用于 elisa;wb

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