Rabbit anti Human Interleukin-7 antibody AHP283B bio-rad,Rabbit anti Human Interleukin-7 antibody

2024-10-22

Rabbit anti Human Interleukin-7 antibody

货号:AHP283B

规格:50 µg

价格:4055

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Interleukin-7

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Biotin

产品介绍
Rabbit anti Human Interleukin-7 antibodyrecognizes human interleukin-7. IL-7 is a 152 amino acid glycoprotein and pleiotropic haematopoietic growth factor, secreted by thymic, splenic and bone marrow stromal/epithelial cells, keratinocytes and follicular dendritic cells. Originally termed pre-B cell factor, due to its involvement in the development of early B cells, IL-7 has since been shown to be mitogenic for early T cells and can also co-stimulate the proliferation of mature T cells in the presence of IL-2 and Concavalin A. IL-7 signals through a transmembrane receptor consisting of the IL-7Ra chain (CD127) and common cytokine gamma chain, triggering a number of non-receptor tyrosine kinase signalling pathways.
产品详情
Target SpeciesHuman
Product FormPurified IgG - lyophilized
ReconstitutionReconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Buffer SolutionPhosphate buffered saline
Antiserum PreparationAntisera to human IL-7 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by affinity chromatography.
Preservative StabilisersPhosphate buffered saline
ImmunogenRecombinant human IL-7 (PHP046).
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml after reconstitution
RegulatoryFor research purposes only
GuaranteeGuaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
ELISA0.52.0 ug/ml
Functional Assays0.-50.08 ug/ml
Western Blotting0.10.2 ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg
文献
1.Lupton, S.D.et al.(1990) Characterization of the human and murine IL-7 genes.J Immunol. 144 (9): 3592-601.2.Fry, T.J. & Mackall, C.L. (2002) Interleukin-7: from bench to clinic.Blood. 99 (11): 3892-904.

技术参数

产品应用 elisa;wb

产品类型 Polyclonal Antibody

推荐用于 elisa;wb

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