Bio-Rad MCM2抗体 AHP2404,Rabbit anti MCM2

2024-10-22

Rabbit anti MCM2

货号:AHP2404

规格:0.1 ml

价格:3383

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:MCM2

物种:人

宿主:驴

抗体亚型:IgG3

荧光染料:Alexa Fluor 488

产品介绍

Rabbit anti MCM2 antibodyrecognizes the nuclear protein mini chromosome maintenance protein 2 (MCM2), a putative DNA replicative helicase and member of the MCM family, critical during the initiation of DNA replication.During the G1 phase of the cell cycle the MCM proteins, part of the pre-replicative complex which assembles at replication origins, undergo phosphorylation by kinases including cyclin dependent kinase (CDK) and Cdc7/Dbf4 (DDK). Phosphorylation of MCM2 occurs on multiple sites, namely Ser27, Ser41, Ser53, Ser108 and Ser139 and results in a conformational change and activation of helicase activity.

产品详情

Target Species

Human

Species Cross-Reactivity

Target SpeciesCross Reactivity

Mouse √

Rat √

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

AntiserumPreparation

Antiserum to MCM2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)

Immunogen

Peptide corresponding to an N-terminal sequence of human MCM2

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

应用

Application Name

Verified

Min Dilution

Max Dilution

Immunofluorescence

1/100

1/1000

Immunohistology - Frozen

1/100

1/1000

Immunohistology - Paraffin

1/100

1/1000

Western Blotting

1/1000

1/10000

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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技术参数

产品应用 C;IF;P;WB

产品类型 Polyclonal Antibody

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