Bio-Rad GATA3抗体 AHP2403,Rabbit anti GATA3-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad GATA3抗体 AHP2403,Rabbit anti GATA3

2024-10-22

Rabbit anti GATA3

货号：AHP2403

规格：50 µg

价格：5483

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：GATA3

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Purified

产品介绍
Rabbit anti GATA3 antibodyrecognizes GATA3, also known as Trans-acting T-cell-specific transcription factor GATA-3 or GATA-binding factor 3. The protein is a member of the GATA family of transcription factors, which has received its name from binding DNA sequences containing the GATA consensus motif 5'-[AT]GATA[AG]-3' (uniprot P15976).GATA3 has been shown to play a critical role in T cell development and the differentiation of T helper 2 (Th2) cells (Hoet al.1991). In addition, GATA3 has been implied to have tissue specific functions such as a regulatory role in the development of the mammary gland epithelium and skin tissue (Chouet al. 2010).Aberrant GATA3 levels have been identified in a variety of cancers including breast cancer. Indeed, GATA3 has become a marker for certain breast cancer types and GATA3 antibodies are commonly used for the immunohistochemical evaluation of breast cancer samples (Kringset al.2014).
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
AntiserumPreparation	Antiserum to GATA3 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein G.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN₃)1 mg/ml Bovine Serum Albumin30% Glycerol
Immunogen	Purified partial recombinant GATA3 protein expressed inE. coli
Approx. Protein Concentrations	IgG concentration 1.0mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Immunocytochemistry	√	1/50	1/500
Immunofluorescence	√	1/50	1/500
Immunohistology - Paraffin¹	√	1/50	1/500
Western Blotting	√		1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹This antibody requires heat-mediated antigen retrieval prior to staining. Bio-Rad recommend citrate buffer pH6.0 for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Western Blotting Rabbit anti GATA3 antibody recognizes a band of approximately 55 kDa in SH-SY5Y cell lysate and SH-SY5Y nuclear extract
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技术参数

产品应用 IC； IF； P*； WB

产品类型 Polyclonal Antibody

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Bio-Rad GATA3抗体 AHP2403,Rabbit anti GATA3

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