Bio-Rad AMPK Alpha 2抗体 AHP2256,Goat anti Human AMPK Alpha 2

2024-10-22

Goat anti Human AMPK Alpha 2

货号:AHP2256

规格:0.1 mg

价格:4463

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:AMPK Alpha 2

物种:人

宿主:山羊

抗体亚型:IgG

荧光染料:Purified

产品介绍

Goat anti Human AMPK alpha 2 antibodyrecognizes human5′-AMP-activated protein kinase catalytic subunit alpha-2, also known as AMPK alpha 2, ACACA kinase, Acetyl-CoA carboxylase kinase or Hydroxymethylglutaryl-CoA reductase kinase, encoded by the PRKAA2 gene. AMPK alpha 2 is a a Serine/threonine-protein kinase and the catalytic subunit of the AMP-activated protein kinase (AMPK), a central metabolic sensor, essential for the regulation of fatty acid and cholesterol synthesis, in response to fluctuations in nutrient and intracellular energy levels.AMPK has been implicated as a suppressor of cell proliferation of both normal and malignant cells. AMPK binding to the PS domain of ULK1, is essential for ULK1-mediated autophagy (Roachet al.2011), and that the phosphorylation of raptor by AMPK, plays an important role in autophagy induction (Eganet al.2011).

产品详情

Target Species

Human

Species Cross-Reactivity

Target Species Cross Reactivity

Bovine Expected from Sequence

Dog Expected from Sequence

Mouse √

Rat √

Pig Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

AntiserumPreparation

Antiserum to human AMPK alpha 2 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin

Immunogen

Synthetic peptide sequence CPLDALNTTKP from the internal region of AMPK alpha 2 (NP_006243.2).

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

1/16000

Western Blotting

1.0

3.0 ug/ml

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • Western Blotting
  • AHP2256 detects a band of approximately 65kDa in Human heart and liver cell lysates.

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Description

Product Code

Applications

Pack Size

Rabbit anti Goat IgG (Fc):FITC

STAR122F

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Rabbit anti Goat IgG (Fc):HRP

STAR122P

C E WB

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技术参数

产品应用 E; WB

产品类型 Polyclonal Antibody

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