Bio-Rad Histone H4 Ac12抗体 AHP2258,Rabbit anti Histone H4 (Ac12)

2024-10-22

Rabbit anti Histone H4 (Ac12)

货号:AHP2258

规格:0.1 ml

价格:4021

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Histone H4 Ac12

物种:Synthetic Peptide

宿主:兔

抗体亚型:IgG

荧光染料:其它

产品介绍

Rabbit anti Histone H4 (Ac12) antibodyrecognizes Histone H4 acetylated at lysine 12. Histone H4 is a 102 amino acid ~12 kDa nuclear transcritional regulator involved in DNA repair, replication and chromosome stability.

产品详情

Target Species

Synthetic Peptide

Species Cross-Reactivity

Target SpeciesCross Reactivity

Human √

Drosophila √

Mouse √

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Serum - liquid

AntiserumPreparation

Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.

Preservative Stabilisers

<0.1% Sodium Azide (NaN3)

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

Western Blotting

1/500

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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文献

1.Turner, B.M.et al.(1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabeling with site-specific antibodies.FEBS Lett. 253 (1-2): 141-5.2.Turner, B.M.et al.(1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains inDrosophilapolytene nuclei.Cell. 69 (2): 375-84.3.Belyaev, N.et al.(1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.Hum Genet. 97 (5): 573-8.4.Tsaprouni, L.G.et al.(2011) Differential patterns of histone acetylation in inflammatory bowel diseases.J Inflamm (Lond). 8 (1): 1.5.Chen, W.Y.et al.(2000) Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation.Proc Natl Acad Sci U SA. 97: 377-82.6.Friis, R.M.et al.(2009) A glycolytic burst drives glucose induction of global histone acetylation by picNuA4 and SAGA.Nucleic Acids Res. 37: 3969-80.7.Ito, K.et al.(2000) Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1beta-induced histone H4 acetylation on lysines 8 and 12.Mol Cell Biol. 20: 6891-903.8.Kobor, M.S.et al(2004) A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin.PLoS Biol. 2: E131.View The Latest Product References

技术参数

产品应用 E; WB

产品类型 Polyclonal Antibody

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