Bio-Rad AHP2049 collagen VI ,Rabbit anti Human collagen VI antibody

2024-10-22

Rabbit anti Human collagen VI antibody

货号:AHP2049

规格:50 µg

价格:8560

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:collagen VI

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human collagen VI antibodydetects mammalian collagen type VI, also known as COL6A, a secreted extracellular matrix protein involved in cellular adhesion.Collagen type specific antibodies are dependent on non-denatured three-dimensional epitopes. Therefore, the reactivity and selectivity of this antibody will be significantly reduced if denatured and reducing conditions of SDS-PAGE and immunblotting are used.
产品详情

Target Species

Human
Species Cross-ReactivityTarget SpeciesCross Reactivity
Bovine
MammalsExpected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to Collagen IV was raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG prepared from whole serum by affinity chromatography.

Buffer Solution

0.02M Potassium Phosphate, 0.15M NaCl

Preservative Stabilisers

0.01% Sodium Azide (NaN3)

Immunogen

Collagen Type VI from Human and Bovine Placenta.
Approx. Protein ConcentrationsTotal protein concentration 1.0 mg/ml

Regulatory

For research purposes only

Guarantee

12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

Immunohistology - Paraffin115.0ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Histology Positive Control TissueHuman breast or lung
数据

Staining of formalin fixed, paraffin embedded human breast with Rabbit anti Human collagen 6 (AHP2049).
相关试剂
DescriptionProduct CodeApplicationsPack Size
Antigen Retrieval Buffer, pH8.0BUF025AIHC(P)500 mL

技术参数

产品应用 P*

产品类型 Polyclonal Antibody

推荐用于 P*

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