Bio-Rad AHP2024 AIF1,Goat anti AIF1 antibody-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad AHP2024 AIF1,Goat anti AIF1 antibody

2024-10-22

Goat anti AIF1 antibody

货号：AHP2024

规格：0.1 mg

价格：5083

产品类型：免疫组化一抗

品牌：Bio-Rad

抗原：AIF1

物种：人

宿主：山羊

抗体亚型：IgG

荧光染料：Purified

产品介绍
Goat anti AIF1 antibodyrecognizes human allograft inflammatory factor 1 (AIF1), otherwise known as Ionized calcium-binding adapter molecule 1 (IBA1). AIF1 is a 147 amino acid ~17kDa actin-binding protein involved in macrophage activation, vascular smooth muscle cell proliferation and activation and T-lymphocyte proliferation (UniProt P55008). Goat anti AIF1 antibody recognizes epitopes at the C-terminal region of AIF1 and is expected to recognize AIF1 isoform 1 and isoform 3 formed by alternative splicing.AIF1 was first identified in rat cardiac allografts with chronic rejection (Utanset al.1995), is expressed by activated monocytes and macrophages (Orsmarket al.2007) and plays a significant role in vascular inflammation (Chenet al.2004)Goat anti AIF1 has been used successfully for the identification of AIF1 expressing cells in an inflammatory model in formalin fixed mouse brain following heat mediated antigen retrieval with sodium citrate buffer by immunohistochemistry (Wanget al.2014).
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Mouse	√
Pig	Expected from Sequence
Rat	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Antiserum Preparation	Antiserum to human AIF1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution	TRIS buffered saline
Preservative Stabilisers	0.02% Sodium Azide (NaN3)0.5% Bovine Serum Albumin
Immunogen	Synthetic peptide sequence C-TGPPAKKAISELP from the C-terminal region of AIF1 (NP_116573.1; NP_001614.3).
Approx. Protein Concentrations	IgG concentration 0.5 mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√	1/32000
Immunohistology - Paraffin¹	√	2.0ug/ml
Western Blotting	√	1.0	3.0ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Tris/EDTA buffer pH9.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Western BlottingAHP2024 detects a band of approximately 16kDa in rat brain cell lysates.
数据
Immunohistochemical staining of formalin fixed, paraffin embedded mouse brain with Goat anti Human AIF1 antibody (AHP2024) following heat mediated antigen retrieval in Tris/EDTA buffer (pH9.0)
相关试剂
Description	Product Code	Applications	Pack Size
Antigen Retrieval Buffer, pH8.0	BUF025A	IHC(P)	500 mL
文献
1. Wang, X.et al.(2014) Pseudoginsenoside-F11 (PF11) exerts anti-neuroinflammatory effects on LPS-activated microglial cells by inhibiting TLR4-mediated TAK1/IKK/NF-κB, MAPKs and Akt signaling pathways.Neuropharmacology. 79: 642-56.2. Kang, D.H.et al.(2020) Effects of Cerebrolysin on Hippocampal Neuronal Death After Pilocarpine-Induced Seizure.Front Neurosci. 14: 568813.3. Wu, C.et al.(2021) E3 ubiquitin ligase Triad1 promotes neuronal apoptosis by regulating the p53-caspase3 pathway after spinal cord injury.Somatosens Mot Res. : 1-8.

技术参数

产品应用 E;P*;WB

产品类型 Polyclonal Antibody

推荐用于 E;P*;WB

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Bio-Rad AHP2024 AIF1,Goat anti AIF1 antibody

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