Bio-Rad AHP1920 CCL25,Rabbit anti Human CCL25 antibody

2024-10-22

Rabbit anti Human CCL25 antibody

货号:AHP1920

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:CCL25

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Human CCL25 antibodyrecognizes human CCL25, otherwise known as TECK (thymus expressed chemokine), a member of the intercrine beta (chemokine CC) family, expressed by thymic stromal cells, which acts as a chemotactic factor for activated macrophages, thymocytes and dendritic cells, and signals through binding to the CCR9 receptor.
产品详情

Target Species

Human

Product Form

Purified IgG - lyophilized
ReconstitutionReconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide

Antiserum Preparation

Antiserum to human CCL25 were raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

None present

Carrier FreeYes

Immunogen

Recombinant human CCL25.
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml after reconstitution

Regulatory

For research purposes only

Guarantee

12 months from date of despatch
存储条件
Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA0.52.0 ug/ml
Functional Assays10.015.0 ug/ml
Immunohistology - Paraffin10.51.0 ug/ml
Western Blotting0.10.2 ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
数据

Formalin fixed, paraffin embedded normal human small bowel stained with Rabbit anti Human CCL25 (AHP1920) following antigen retrieval with proteinase k.
相关试剂
DescriptionProduct CodeApplicationsPack Size
TidyBlot Western Blot Detection Reagent:HRPSTAR209PWB0.5 mL
Antigen Retrieval Buffer, pH8.0BUF025AIHC(P)500 mL

技术参数

产品应用 E;FN;P*;WB

产品类型 Polyclonal Antibody

推荐用于 E;FN;P*;WB

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