产品介绍 |
| Rabbit anti Human WNT-3a antibodyrecognizes human WNT-3a, a secreted protein and member of the Wnt family of signalling proteins, acting as a ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3a plays key roles in many developmental and cell fate decisions, including embryonic patterning, CNS development, and cytoskeletal formation.Wnt-3a acts as a promoter of the proliferation of ventral midbrain dopaminergic neuron precursor cells, and has been implicated in the progressive differentiation of anterior neural cells, as well as regulating the survival and expansion of neural progenitors derived from human embryonic stem cells. Wnt3a acts upstream of the Notch signalling pathway, in the regulation of somitogenesis, controlled by a segmentation clock, consisting of molecular oscillators in the Notch, Wnt3a and Fgf8 pathways(Aulehlaet al.2003). |
产品详情 |
Target Species | Human |
Product Form | Purified IgG - lyophilized |
Reconstitution | Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. |
AntiserumPreparation | Antiserum to human WNT-3a were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Carrier Free | Yes |
Immunogen | Recombinant human WNT-3a. |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml after reconstitution |
Regulatory | For research purposes only |
Guarantee | 6 months from date of despatch |
存储条件 |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 0.5 | 2.0ug/ml |
Immunohistology -Paraffin1 | √ | | |
Western Blotting | √ | 0.1 | 0.2ug/ml |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.- ELISA
- AHP2188 may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with (AHP2188B) as the detection antibody.
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可用二抗 |
Description | Product Code | Applications | Pack Size |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
相关产品 |
Description | Product Code | Applications | Pack Size |
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml |
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
文献 |
| 1.Lai, Y.H.et al.(2011) SOX4 interacts with plakoglobin in a Wnt3a-dependent manner in prostate cancer cells.BMC Cell Biol. 12: 50.2.Yan, L.et al.(2011) Activation of the canonical Wnt/β-catenin pathway in ATF3-induced mammary tumors.PLoS One. 6:e16515. |
QQ:1749072012