Bio-Rad TIMP-1抗体 AHP2187,Rabbit anti Human TIMP-1

2024-10-22

Rabbit anti Human TIMP-1

货号:AHP2187

规格:0.1 mg

价格:4063

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:TIMP-1

物种:人

宿主:兔

抗体亚型:IgG

荧光染料:Purified

产品介绍

Rabbit anti Human TIMP-1 antibodyrecognizes human TIMP-1 (Tissue Inhibitor of Metalloproteinase -1), a secreted glycoprotein and member of the protease inhibitor I35 (TIMP) family, which is expressed by a wide range of cells, including microvascular endothelial cells, smooth muscle cells, mesangial cells, and some fibroblasts. TIMP-1 acts as a natural extracellular inhibitor of MMPs (matrix metalloproteinases), a group of peptidases involved in degradation of the extracellular matrix, and is also involved in erythropoiesis, through the simulation of the proliferation/differentiation of erythroid progenitors. A correlation between localized concentrations of TIMP-1 and TIMP-3, may determine cell fate, and that increased expression of TIMP-1, may act as an inhibitor/suppressor of invasive cell growth (Sternlicht and Werb 2001)

产品详情

Target Species

Human

Product Form

Purified IgG - lyophilized

Reconstitution

Reconstitute with 0.1ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

AntiserumPreparation

Antiserum to human TIMP-1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

None present

Carrier Free

Yes

Immunogen

Recombinant human TIMP-1.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml after reconstitution

Regulatory

For research purposes only

Guarantee

12 months from date of despatch

存储条件

Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

0.5

2.0ug/ml

Functional Assays

Immunohistology - Paraffin1

Western Blotting

0.1

0.2ug/ml

1This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
  • ELISA
  • This purified human TIMP-1 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with ourbiotinylated human TIMP-1 antibody(AHP2187B) as the detection reagent.

可用二抗

Description

Product Code

Applications

Pack Size

Goat anti Rabbit IgG (Fc):Biotin

STAR121B

E WB

1 mg

Goat anti Rabbit IgG (Fc):FITC

STAR121F

F

1 mg

Goat anti Rabbit IgG (Fc):HRP

STAR121P

E WB

1 mg

Goat anti Rabbit IgG (H/L):HRP

STAR124P

C E WB

1 mg

Sheep anti Rabbit IgG:FITC

STAR34B

C F

1 mg

Sheep anti Rabbit IgG:RPE

STAR35A

F

1 ml

相关产品

Description

Product Code

Applications

Pack Size

Antigen Retrieval Buffer, pH8.0

BUF025A

P

500 ml

TidyBlot Western Blot Detection Reagent:HRP

STAR209P

WB*

0.5 ml

技术参数

产品应用 E ;FN ;P*; WB

产品类型 Polyclonal Antibody

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