产品介绍

产品详情

Target Species

Human

Product Form

Purified IgG conjugated to Biotin - lyophilized

Reconstitution

Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albuminCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

AntiserumPreparation

Antiserum to human IL-1RA were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

None present

Carrier Free

Yes

Immunogen

Recombinant human IL-1RA (PHP080)

Approx. Protein Concentrations

IgG concentration 0.1mg/ml after reconstitution

Regulatory

For research purposes only

Guarantee

6 months from date of despatch

存储条件

应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

√

0.25

1.0ug/ml

Western Blotting

√

0.1

0.2ug/ml

• ELISA
• This biotinylated human IL-1RA antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with apurified human IL-1RA antibody(AHP2184) as the capture reagent andrecombinant human IL-1RA(PHP080) as the standard.
• Western Blotting
• This biotinylated human IL-1RA antibody may be used in Western Blotting applications under either reducing or non-reducing conditions withrecombinant human IL-1RA(PHP080) as the positive control.